Rabbit anti-Human CLEC10A Polyclonal Antibody | anti-CLEC10A antibody

CLEC10A, NT (CLEC10A, CLECSF13, CLECSF14, HML, C-type lectin domain family 10 member A, C-type lectin superfamily member 14, Macrophage lectin 2, CD301) (Azide free) (HRP)

Cat. Num. AAA6286562

• Gene Names: CLEC10A; HML; MGL; HML2; CD301; CLECSF13; CLECSF14
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLEC10A; Polyclonal Antibody; NT (CLEC10A; CLECSF13; CLECSF14; HML; C-type lectin domain family 10 member A; C-type lectin superfamily member 14; Macrophage lectin 2; CD301) (Azide free) (HRP); CD301; anti-CLEC10A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CLEC10A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CLEC10A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CLEC10A.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CLEC10A Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CLEC10A antibody detected the CLEC10A protein (arrow).)

Flow Cytometry (FC/FACS)

(CLEC10A Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CLEC10A antibody

CLEC10A encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-CLEC10A antibody

Antibodies; CD Markers

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Iijima, M., et al. Neurology 73(17):1348-1352(2009). van Vliet, S.J., et al. PLoS Pathog. 5 (10), E1000625 (2009): Suzuki, N., et al. J. Immunol. 156(1):128-135(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 33667103
• NCBI GeneID: 10462
• NCBI Accession #: NP_878910.1; NP_006335.2
• NCBI GenBank Nucleotide #: NM_182906.2
• UniProt Accession #: Q8IUN9; Q14538; Q6PIW3; A8K8J8
• Molecular Weight: 28,877 Da
• NCBI Official Full Name: C-type lectin domain family 10 member A isoform 1
• NCBI Official Synonym Full Names: C-type lectin domain family 10 member A
• NCBI Official Symbol: CLEC10A
• NCBI Official Synonym Symbols: HML; MGL; HML2; CD301; CLECSF13; CLECSF14
• NCBI Protein Information: C-type lectin domain family 10 member A
• UniProt Protein Name: C-type lectin domain family 10 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC10A
• UniProt Synonym Gene Names: CLECSF13; CLECSF14; HML
• UniProt Entry Name: CLC10_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CLEC10A: Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbohydrate binding

Biological Process: innate immune response; endocytosis