Rabbit Cleaved-ADAM12 Polyclonal Antibody | anti-ADAM12 antibody

Cleaved-ADAM12 (E208) Polyclonal Antibody

Cat. Num. AAA3013915

• Gene Names: ADAM12; MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cleaved-ADAM12; Polyclonal Antibody; Cleaved-ADAM12 (E208) Polyclonal Antibody; Disintegrin and metalloproteinase domain-containing protein 12; ADAM 12; Meltrin-alpha; ADAM12; MLTN; UNQ346; PRO545; anti-ADAM12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cleaved-ADAM12 (E208) polyclonal antibody detects endogenous levels of Cleaved-ADAM12 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 909

• Applicable Applications for anti-ADAM12 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 208-259 of Human ADAM12.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cleaved-ADAM12 (E208) polyclonal antibody at 1:500 dilutionLane1:U-87MG whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:The Embryo tissue lysate of Mouse (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug))

Related Product Information for anti-ADAM12 antibody

ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNF (tumor necrosis factor-alpha), and modulation of the neurogenic function of Notch and Delta. ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factorlike domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone and muscle, and catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAM12 (Meltrin-alpha) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion.

NCBI and Uniprot Product Information

• NCBI GI #: 229463008
• NCBI GeneID: 8038
• UniProt Accession #: O43184
• Molecular Weight: ~ 77, 99kDa
• NCBI Official Full Name: Disintegrin and metalloproteinase domain-containing protein 12
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 12
• NCBI Official Symbol: ADAM12
• NCBI Official Synonym Symbols: MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 12
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 12
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM12
• UniProt Synonym Gene Names: MLTN; ADAM 12
• UniProt Entry Name: ADA12_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

Uniprot Description

ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.

Protein type: Protease; Membrane protein, integral; EC 3.4.24.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; mitochondrion; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; proteolysis; cell adhesion; myoblast fusion