Rabbit anti-Human Cleavage Stimulation Factor 2 Polyclonal Antibody | anti-CSTF2 antibody

Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (FITC)

Cat. Num. AAA6470758

• Gene Names: CSTF2; CstF-64
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Cleavage Stimulation Factor 2; Polyclonal Antibody; NT (Cleavage Stimulation Factor 64kD Subunit; CSTF 64kD Subunit; CF-1 64kD Subunit; CstF-64; CSTF2) (FITC); anti-CSTF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CSTF2.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CSTF2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 31-60 from the N-terminal region of human Cleavage Stimulation Factor 2 (P33240).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NCI-H460 cell line lysates (35ug/lane) usingMBS626293. CSTF2(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CSTF2 antibody

Antibodies; Proteins

References

1. Takagaki, Y., et al., Mol. Cell 2(6):761-771 (1998). 2. Martincic, K., et al., Proc. Natl. Acad. Sci. USA 95(19):11095-11100 (1998). 3. Takagaki, Y., et al., Cell 87(5):941-952 (1996). 4. Takagaki, Y., et al., Nature 372(6505):471-474 (1994). 5. Takagaki, Y., et al., Proc. Natl. Acad. Sci. USA 89(4):1403-1407 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4557493
• NCBI GeneID: 1478
• NCBI Accession #: NP_001316
• NCBI GenBank Nucleotide #: NM_001325
• UniProt Accession #: P33240; Q5H951; Q6LA74; Q8N502
• Molecular Weight: 59,251 Da
• NCBI Official Full Name: cleavage stimulation factor subunit 2
• NCBI Official Synonym Full Names: cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• NCBI Official Symbol: CSTF2
• NCBI Official Synonym Symbols: CstF-64
• NCBI Protein Information: cleavage stimulation factor subunit 2; CF-1 64 kDa subunit; CSTF 64 kDa subunit; betaCstF-64 variant 2; cleavage stimulation factor 64 kDa subunit; cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD
• UniProt Protein Name: Cleavage stimulation factor subunit 2
• Protein Family: Cleavage stimulation factor
• UniProt Gene Name: CSTF2
• UniProt Synonym Gene Names: CSTF 64 kDa subunit; CstF-64
• UniProt Entry Name: CSTF2_HUMAN

Uniprot Description

CSTF2: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing; mRNA polyadenylation; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; mRNA cleavage