Rabbit anti-Human CLDN16 Polyclonal Antibody | anti-CLDN16 antibody

CLDN16 Polyclonal Antibody

Cat. Num. AAA9132828

• Gene Names: CLDN16; HOMG3; PCLN1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: CLDN16; Polyclonal Antibody; CLDN16 Polyclonal Antibody; HOMG3; PCLN1; anti-CLDN16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 305

• Applicable Applications for anti-CLDN16 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: A synthetic peptide of human CLDN16
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CLDN16 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Related Product Information for anti-CLDN16 antibody

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28.

Product Categories/Family for anti-CLDN16 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 6685318
• NCBI GeneID: 10686
• UniProt Accession #: Q9Y5I7
• Molecular Weight: 34kDa
• NCBI Official Full Name: Claudin-16
• NCBI Official Synonym Full Names: claudin 16
• NCBI Official Symbol: CLDN16
• NCBI Official Synonym Symbols: HOMG3; PCLN1
• NCBI Protein Information: claudin-16
• UniProt Protein Name: Claudin-16
• Protein Family: Claudin
• UniProt Gene Name: CLDN16
• UniProt Synonym Gene Names: PCLN1; PCLN-1

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. [provided by RefSeq, Jun 2010]

Uniprot Description

Claudin-16: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Defects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3); also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia. Belongs to the claudin family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: tight junction

Molecular Function: identical protein binding; magnesium ion transmembrane transporter activity; protein binding

Biological Process: calcium-independent cell-cell adhesion; cellular metal ion homeostasis; excretion

Disease: Hypomagnesemia 3, Renal