Rabbit anti-Mouse CLCNKB Polyclonal Antibody | anti-CLCNKB antibody

CLCNKB, NT (CLCNKB, Chloride channel protein ClC-Kb, ClC-K2) (Azide free) (HRP)

Cat. Num. AAA6286408

• Gene Names: CLCNKB; CLCKB; ClC-K2; ClC-Kb
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLCNKB; Polyclonal Antibody; NT (CLCNKB; Chloride channel protein ClC-Kb; ClC-K2) (Azide free) (HRP); ClC-K2; anti-CLCNKB antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CLCNKB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CLCNKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human CLCNKB.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CLCNKB Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the CLCNKB antibody detected the CLCNKB protein (arrow).)

Related Product Information for anti-CLCNKB antibody

The protein encoded by this gene is a member of the family of voltage-gated chloride channels. Chloride channels have several functions, including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. This gene is expressed predominantly in the kidney and may be important for renal salt reabsorption. Mutations in this gene are associated with autosomal recessive Bartter syndrome type 3 (BS3). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-CLCNKB antibody

Antibodies; Ion Channel

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Yu, Y., et al. Clin. Genet. 77(2):155-162(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Brochard, K., et al. Nephrol. Dial. Transplant. 24(5):1455-1464(2009). Sile, S., et al. J. Hypertens. 27(2):298-304(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 155969705
• NCBI GeneID: 1188
• NCBI Accession #: NP_000076.2
• NCBI GenBank Nucleotide #: NM_000085.4
• UniProt Accession #: P51801; Q5T5Q7; Q5T5Q8; B3KUY3
• Molecular Weight: 56,998 Da
• NCBI Official Full Name: chloride channel protein ClC-Kb isoform 1
• NCBI Official Synonym Full Names: chloride voltage-gated channel Kb
• NCBI Official Symbol: CLCNKB
• NCBI Official Synonym Symbols: CLCKB; ClC-K2; ClC-Kb
• NCBI Protein Information: chloride channel protein ClC-Kb
• UniProt Protein Name: Chloride channel protein ClC-Kb
• Protein Family: Chloride channel protein
• UniProt Gene Name: CLCNKB
• UniProt Synonym Gene Names: Chloride channel Kb
• UniProt Entry Name: CLCKB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the family of voltage-gated chloride channels. Chloride channels have several functions, including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. This gene is expressed predominantly in the kidney and may be important for renal salt reabsorption. Mutations in this gene are associated with autosomal recessive Bartter syndrome type 3 (BS3). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. Ref.6

Subunit structure: Interacts with BSND. Forms heteromers with BSND in the thick ascending limb of Henle and more distal segments

By similarity.

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Expressed predominantly in the kidney. Ref.6

Involvement in disease: Bartter syndrome 3 (BS3) [MIM:607364]: An autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9Bartter syndrome 4B (BS4B) [MIM:613090]: A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.Note: The disease is caused by mutations affecting distinct genetic loci, including the gene represented in this entry. Loss-of-function of both CLCNKA and CLCNKB results in the disease phenotype (Ref.8). Ref.7 Ref.8

Miscellaneous: Compared with CLCNKA/BSND, CLCNKB/BSND is more sensitive to pH and less responsive to Ca2+.

Sequence similarities: Belongs to the chloride channel (TC 2.A.49) family. CLCNKB subfamily. [View classification]Contains 2 CBS domains.