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Western Blot (WB) (CK073 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CK073 antibody detected the CK073 protein (arrow).)

Rabbit CK073 Polyclonal Antibody | anti-CK073 antibody

CK073, NT (C11orf73, Protein Hikeshi) (FITC)

Gene Names
C11orf73; L7RN6; OPI10; HSPC138; HSPC179; Hikeshi
Reactivity
Hamster, Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CK073; Polyclonal Antibody; NT (C11orf73; Protein Hikeshi) (FITC); C11orf73; Protein Hikeshi; anti-CK073 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Hamster, Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-CK073 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CK073 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human CK073.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CK073 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CK073 antibody detected the CK073 protein (arrow).)

Western Blot (WB) (CK073 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CK073 antibody detected the CK073 protein (arrow).)

Western Blot (WB)

(CK073 Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the CK073 antibody detected the CK073 protein (arrow).)

Western Blot (WB) (CK073 Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the CK073 antibody detected the CK073 protein (arrow).)

Immunohistochemistry (IHC)

(CK073 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CK073 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (CK073 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CK073 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CK073 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (CK073 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CK073 antibody
Required for organization and/or function of the secretory apparatus in Clara cells in lung (By similarity).
Product Categories/Family for anti-CK073 antibody
References
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,628 Da
NCBI Official Full Name
protein Hikeshi
NCBI Official Synonym Full Names
chromosome 11 open reading frame 73
NCBI Official Symbol
C11orf73
NCBI Official Synonym Symbols
L7RN6; OPI10; HSPC138; HSPC179; Hikeshi
NCBI Protein Information
protein Hikeshi
UniProt Protein Name
Protein Hikeshi
UniProt Gene Name
C11orf73
UniProt Entry Name
HIKES_HUMAN

Uniprot Description

C11orf73: Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 11q14.2

Cellular Component: intracellular; cell junction; cytosol; nucleus

Molecular Function: Hsp70 protein binding; protein transporter activity

Biological Process: protein transport; protein import into nucleus; Golgi organization and biogenesis; lung development

Research Articles on CK073

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Product Notes

The CK073 c11orf73 (Catalog #AAA6286253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CK073, NT (C11orf73, Protein Hikeshi) (FITC) reacts with Hamster, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CK073 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CK073 c11orf73 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK073, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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