Rabbit anti-Rat, Mouse CISH Polyclonal Antibody | anti-CISH antibody

CISH polyclonal antibody

Cat. Num. AAA3005500

• Gene Names: CISH; CIS; G18; SOCS; CIS-1; BACTS2
• Reactivity: Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Synonyms: CISH; Polyclonal Antibody; CISH polyclonal antibody; Cytokine-inducible SH2-containing protein; CIS; CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS; G18; anti-CISH antibody

• Host: Rabbit
• Reactivity: Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CISH polyclonal antibody detects endogenous levels of CISH protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-CISH antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human CISH.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of CISH polyclonal antibody at 1:500 dilution Lane1:The Liver tissue lysate of Mouse(40ug) Lane2:The Kidney tissue lysate of Rat(40ug) Lane3:The Liver tissue lysate of Rat(40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human Liver cancer using CISH antibody at dilution of 1:50.)

Related Product Information for anti-CISH antibody

The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway. The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. CISH/CIS1, the first described member of the SOCS family, is induced by a number of cytokines including IL-2, IL-3, GM-CSF, and EPO. The CISH protein appears as a doublet around 32 and 37 kDa, the nature of which is unknown. CISH binds to phosphorylated cytokine receptors and can inhibit Stat5 activity. Expression of CISH is regulated by Stat5, thereby providing feedback modulation. Transgenic mice overexpressing CISH display phenotypes similar to Stat5 knockouts, including defects in mammary gland development and in T and NK cell regulation. Research studies have shown that polymorphisms within the CISH gene are associated with susceptibility to infectious diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 13124022
• NCBI GeneID: 1154
• UniProt Accession #: Q9NSE2; Q9NS38; Q9Y5R1; B2R9N1; G5E9R1
• Molecular Weight: 30,734 Da
• NCBI Official Full Name: Cytokine-inducible SH2-containing protein
• NCBI Official Synonym Full Names: cytokine inducible SH2-containing protein
• NCBI Official Symbol: CISH
• NCBI Official Synonym Symbols: CIS; G18; SOCS; CIS-1; BACTS2
• NCBI Protein Information: cytokine-inducible SH2-containing protein; suppressor of cytokine signaling; cytokine-inducible inhibitor of signaling type 1B
• UniProt Protein Name: Cytokine-inducible SH2-containing protein
• Protein Family: Cytokine-inducible SH2-containing protein
• UniProt Gene Name: CISH
• UniProt Synonym Gene Names: G18; CIS; SOCS
• UniProt Entry Name: CISH_HUMAN

NCBI Description

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

CISH: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate- recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR). By a subset of cytokines including EPO/erythropoietin. Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; protein kinase inhibitor activity

Biological Process: cytokine and chemokine mediated signaling pathway; protein kinase C activation; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; regulation of cell growth; negative regulation of JAK-STAT cascade

Disease: Mycobacterium Tuberculosis, Susceptibility To; Bacteremia, Susceptibility To, 2; Malaria, Susceptibility To