Rabbit anti-Rat Ciliary Neurotropic Factor Polyclonal Antibody | anti-Cntf antibody

Ciliary Neurotropic Factor (CNTF) (Biotin)

Cat. Num. AAA610837

• Gene Names: Cntf; Cntf
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Puri ed by af nity chromatography and then biotinylated.
• Synonyms: Ciliary Neurotropic Factor; Polyclonal Antibody; Ciliary Neurotropic Factor (CNTF) (Biotin); Anti -Ciliary Neurotropic Factor (CNTF) (Biotin); anti-Cntf antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CNTF
• Purity/Purification: Affinity Purified; Puri ed by af nity chromatography and then biotinylated.
• Form/Format: Lyophilized from sterile- ltered PBS solution.

• Applicable Applications for anti-Cntf antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: ELISA: To detect rat CNTF by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15- 0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant rat CNTF is 1.5 - 3.0ng/lane, under either reducing or non-reducing conditions.; Western Blot: To detect rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat CNTF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
• Immunogen: Highly pure (>98%) recombinant rat CNTF (rat CNTF).
• Country of Origin: USA
• Reconstitution: The biotinylated antibody should be reconstituted to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4 degree C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at-20 degree C. Avoid more than one freeze-thaw cycle.
• Preparation and Storage: -20 degree C; Stability: The lyophilized antibody is stable for at least 6 months when kept at-20 degree C.

Product Categories/Family for anti-Cntf antibody

Antibodies; Abs to Neurotrophins

NCBI and Uniprot Product Information

• NCBI GI #: 6978675
• NCBI GeneID: 25707
• NCBI Accession #: NP_037298.1
• NCBI GenBank Nucleotide #: NM_013166.1
• UniProt Accession #: P20294
• Molecular Weight: 22,854 Da
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: Cntf
• NCBI Official Synonym Symbols: Cntf
• NCBI Protein Information: ciliary neurotrophic factor; ciliary neurotropic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: Cntf
• UniProt Entry Name: CNTF_RAT

NCBI Description

a cytokine involved in neuronal degeneration and adipocyte gene expression [RGD]

Uniprot Description

Function: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Subcellular location: Cytoplasm.

Tissue specificity: Nervous system.

Sequence similarities: Belongs to the CNTF family.