Rabbit anti-Human CIDEC Polyclonal Antibody | anti-CIDEC antibody

CIDEC, ID (CIDEC, FSP27, Cell death activator CIDE-3, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog) (FITC)

Cat. Num. AAA6286176

• Gene Names: CIDEC; FSP27; CIDE-3; FLJ20871
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CIDEC; Polyclonal Antibody; ID (CIDEC; FSP27; Cell death activator CIDE-3; Cell death-inducing DFFA-like effector protein C; Fat-specific protein FSP27 homolog) (FITC); Fat-specific protein FSP27 homolog; anti-CIDEC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CIDEC antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CIDEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-126 amino acids from the Central region of human CIDEC.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CIDEC Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the CIDEC antibody detected the CIDEC protein (arrow).)

Related Product Information for anti-CIDEC antibody

DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis. A novel family of cell death-inducing DFF45 (MIM 601882)-like effectors (CIDEs), including CIDEC, can also promote apoptosis (Liang et al., 2003 [PubMed 12429024]).

Product Categories/Family for anti-CIDEC antibody

Antibodies; Apoptosis

References

Ito, M., et al. J. Lipid Res. 51(7):1676-1684(2010). Nian, Z., et al. J. Biol. Chem. 285(13):9604-9615(2010). Hall, A.M., et al. Obesity (Silver Spring) 18(2):417-419(2010). Rubio-Cabezas, O., et al. EMBO Mol Med 1(5):280-287(2009). Magnusson, B., et al. Metab. Clin. Exp. 57(9):1307-1313(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 23943904
• NCBI GeneID: 63924
• NCBI Accession #: NP_071377.2
• NCBI GenBank Nucleotide #: NM_022094.3
• UniProt Accession #: Q96AQ7; Q67DW9; Q9GZY9
• Molecular Weight: 26,754 Da
• NCBI Official Full Name: cell death activator CIDE-3 isoform 3
• NCBI Official Synonym Full Names: cell death-inducing DFFA-like effector c
• NCBI Official Symbol: CIDEC
• NCBI Official Synonym Symbols: FSP27; CIDE-3; FLJ20871
• NCBI Protein Information: cell death activator CIDE-3; OTTHUMP00000158645; OTTHUMP00000207602; fat specific protein 27
• UniProt Protein Name: Cell death activator CIDE-3
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEC
• UniProt Synonym Gene Names: FSP27
• UniProt Entry Name: CIDEC_HUMAN

NCBI Description

This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq]

Uniprot Description

CIDEC: May act as a CEBPB coactivator in white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. In omental adipose tissue of obese patients matched for BMI, expression levels tend to correlate with insulin sensitivity. Expression is increased 2-3 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle. This effect is not significant in subcutaneous adipose tissue (PubMed:18509062). In subcutaneous adipose tissue of diabetic patients, tends to negatively correlate with body mass index and total fat mass, independently of insulin sensitivity (PubMed:18334488). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytosol; lipid particle

Biological Process: apoptosis; regulation of apoptosis

Disease: Lipodystrophy, Familial Partial, Type 5