Western Blot (WB)
(CIAO1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CIAO1 antibody detected the CIAO1 protein (arrow).)
Rabbit anti-Human CIAO1 Polyclonal Antibody | anti-CIAO1 antibody
CIAO1, ID (CIAO1, WDR39, Probable cytosolic iron-sulfur protein assembly protein CIAO1, WD repeat-containing protein 39) (PE)
Gene Names
CIAO1; CIA1; WDR39
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CIAO1; Polyclonal Antibody; ID (CIAO1; WDR39; Probable cytosolic iron-sulfur protein assembly protein CIAO1; WD repeat-containing protein 39) (PE); WD repeat-containing protein 39; anti-CIAO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CIAO1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CIAO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the Central region of human CIAO1.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(CIAO1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CIAO1 antibody detected the CIAO1 protein (arrow).)
Western Blot (WB)
(CIAO1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CIAO1 antibody detected the CIAO1 protein (arrow).)
Immunohistochemistry (IHC)
(CIAO1 Antibody (Center) (AP14244c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CIAO1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CIAO1 Antibody (Center) (AP14244c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CIAO1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-CIAO1 antibody
CIAO1 is an essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1.
Product Categories/Family for anti-CIAO1 antibody
References
Srinivasan, V., et al. Structure 15(10):1246-1257(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Higa, L.A., et al. Nat. Cell Biol. 8(11):1277-1283(2006). Johnstone, R.W., et al. J. Biol. Chem. 273(18):10880-10887(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,840 Da
NCBI Official Full Name
probable cytosolic iron-sulfur protein assembly protein CIAO1
NCBI Official Synonym Full Names
cytosolic iron-sulfur assembly component 1
NCBI Official Symbol
CIAO1
NCBI Official Synonym Symbols
CIA1; WDR39
NCBI Protein Information
probable cytosolic iron-sulfur protein assembly protein CIAO1; WD repeat domain 39; WD repeat-containing protein 39; WD40 protein Ciao1; cytosolic iron-sulfur protein assembly 1 homolog
UniProt Protein Name
Probable cytosolic iron-sulfur protein assembly protein CIAO1
Protein Family
UniProt Gene Name
CIAO1
UniProt Entry Name
CIAO1_HUMAN
Uniprot Description
CIAO1: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Belongs to the WD repeat CIA1 family.
Chromosomal Location of Human Ortholog: 2q11.2
Biological Process: regulation of transcription from RNA polymerase II promoter; iron-sulfur cluster assembly; positive regulation of cell proliferation; chromosome segregation
Research Articles on CIAO1
Similar Products
Product Notes
The CIAO1 ciao1 (Catalog #AAA6286106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CIAO1, ID (CIAO1, WDR39, Probable cytosolic iron-sulfur protein assembly protein CIAO1, WD repeat-containing protein 39) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CIAO1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CIAO1 ciao1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CIAO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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