Rabbit anti-Human CHUK / IKKA / IKK Alpha Polyclonal Antibody | anti-CHUK antibody

Rabbit Polyclonal to Human CHUK / IKKA / IKK Alpha

Cat. Num. AAA248801

• Gene Names: CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: CHUK / IKKA / IKK Alpha; Polyclonal Antibody; Rabbit Polyclonal to Human CHUK / IKKA / IKK Alpha; Anti-CHUK / IKKA / IKK Alpha Antibody (aa716-734) IHC-plus; CHUK; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; Ikb kinase alpha; IKK-A; IKBKA; IKK1; NFKBIKA; I-kappa-B kinase 1; IkappaB kinase; IKK Alpha; IKK-alpha; IKKA; Transcription factor 16; IkappaBalpha Kinase; IkB kinase alpha subunit; IKK-a kinase; Human CHUK; anti-CHUK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: peptide corresponding to amino acids 716 to 734 of human IKKa, which differs from corresponding murine sequence by four amino acids
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Sequence Length: 745

• Applicable Applications for anti-CHUK antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: ICC, IF (10 ug/ml), IHC-P (1:100), WB (1:1000 - 1:2000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CHUK / IKK-Alpha.
• Immunogen Type: Synthetic peptide
• Immunogen: CHUK / IKKA / IKK Alpha antibody was raised against synthetic peptide from human CHUK / IKK-Alpha.
• Antigen Modification: aa716-734
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-CHUK / IKKA / IKK Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of IKK alpha in Jurkat cells with IKK alpha antibody at 10 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1ug/ml.)

Western Blot (WB)

(Western blot of IKKa in HeLa whole cell lysate with IKKa antibody at 1:1000 dilution.)

Related Product Information for anti-CHUK antibody

IKKalpha, an IkappaB -type protein kinase, is a member of the multimeric IkappaB kinase complex. This complex, often consisting of IKKalpha/IKKbeta heterodimers plus regulatory IKKgamma subunits, phosphorylates IkappaB proteins, marking them for destruction and thereby allowing activation of NFkappaB and downstream nuclear genes. The constitutively expressed IKKalpha protein contains a N-terminal kinase domain, a central leucine zipper motif, and a C-terminal helix-loop-helix motif.

Product Categories/Family for anti-CHUK antibody

Family: Protein Kinase; Subfamily: IKAPPAB

NCBI and Uniprot Product Information

• NCBI GI #: 62241001
• NCBI GeneID: 1147
• NCBI Accession #: NP_001269.3
• NCBI GenBank Nucleotide #: NM_001278.3
• UniProt Accession #: O15111; O14666; Q13132; Q5W0I4; Q92467
• Molecular Weight: 84,640 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit alpha
• NCBI Official Synonym Full Names: conserved helix-loop-helix ubiquitous kinase
• NCBI Official Symbol: CHUK
• NCBI Official Synonym Symbols: IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: CHUK
• UniProt Synonym Gene Names: IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
• UniProt Entry Name: IKKA_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.

Protein type: Kinase, protein; Protein kinase, Other; EC 2.7.11.10; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 10q24-q25

Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol

Molecular Function: protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; ATP binding; protein kinase activity

Biological Process: skeletal muscle contraction; lactation; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to toxin; response to lipopolysaccharide; osteoclast differentiation; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; Rho protein signal transduction; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; morphogenesis of an epithelial sheet; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; response to drug; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; response to virus; MyD88-independent toll-like receptor signaling pathway; mammary gland epithelial cell proliferation; response to amino acid stimulus; toll-like receptor 2 signaling pathway; odontogenesis of dentine-containing teeth; MyD88-dependent toll-like receptor signaling pathway; response to hydroperoxide; toll-like receptor signaling pathway; innate immune response; striated muscle cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation

Disease: Cocoon Syndrome