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Western Blot (WB) (All lanes : Anti-CHTF8 Antibody (N-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human CHTF8 Polyclonal Antibody | anti-CHTF8 antibody

CHTF8 Antibody (N-Term)

Gene Names
CHTF8; CTF8; DERPC
Reactivity
Human
Applications
Western Blot
Synonyms
CHTF8; Polyclonal Antibody; CHTF8 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Chromosome transmission fidelity protein 8 homolog isoform 2; Decreased expression in renal and prostate cancer protein; DERPC; anti-CHTF8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This CHTF8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-33 amino acids from human CHTF8.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
524
Applicable Applications for anti-CHTF8 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:2000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-CHTF8 Antibody (N-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-CHTF8 Antibody (N-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CHTF8 antibody
Potential tumor suppressor. Inhibits prostate tumor cell growth, when overexpressed.
References
Bechtel S.,et al.BMC Genomics 8:399-399(2007). Martin J.,et al.Nature 432:988-994(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Sun M.,et al.Mol. Med. 8:655-663(2002). Green A.R.,et al.Breast Cancer Res. Treat. 113:59-66(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51391
NCBI Official Full Name
chromosome transmission fidelity protein 8 homolog isoform X1
NCBI Official Synonym Full Names
chromosome transmission fidelity factor 8
NCBI Official Symbol
CHTF8
NCBI Official Synonym Symbols
CTF8; DERPC
NCBI Protein Information
chromosome transmission fidelity protein 8 homolog
UniProt Protein Name
Chromosome transmission fidelity protein 8 homolog isoform 2
UniProt Gene Name
CHTF8
UniProt Synonym Gene Names
DERPC
UniProt Entry Name
CTF8A_HUMAN

NCBI Description

This gene encodes a short protein that forms part of the Ctf18 replication factor C (RFC) complex that occurs in both yeast and mammals. The heteroheptameric RFC complex plays a role in sister chromatid cohesion and may load the replication clamp PCNA (proliferating cell nuclear antigen) onto DNA during DNA replication and repair. This gene is ubiquitously expressed and has been shown to have reduced expression in renal and prostate tumors. Alternatively spliced transcript variants have been described. This gene has a pseudogene on chromosome X. [provided by RefSeq, Jan 2010]

Uniprot Description

DERPC: Potential tumor suppressor. Inhibits prostate tumor cell growth, when overexpressed. Belongs to the DERPC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; DNA replication

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: cell cycle; DNA replication

Research Articles on CHTF8

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Product Notes

The CHTF8 chtf8 (Catalog #AAA9216148) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHTF8 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHTF8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the CHTF8 chtf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHTF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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