Rabbit anti-Human, Mouse CHSY1 Polyclonal Antibody | anti-CHSY1 antibody

CHSY1, ID (CHSY1, CHSY, CSS1, KIAA0990, Chondroitin sulfate synthase 1, Chondroitin glucuronyltransferase 1, Chondroitin synthase 1, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1, N-acetylgalactosaminyl-proteo

Cat. Num. AAA6286011

• Gene Names: CHSY1; CHSY; CSS1; TPBS; ChSy-1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHSY1; Polyclonal Antibody; ID (CHSY1; CHSY; CSS1; KIAA0990; Chondroitin sulfate synthase 1; Chondroitin glucuronyltransferase 1; Chondroitin synthase 1; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1; N-acetylgalactosaminyl-proteo; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1; N-acetylgalactosaminyltransferase 1; anti-CHSY1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CHSY1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CHSY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-533 amino acids from the Central region of human CHSY1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHSY1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CHSY1 antibody detected the CHSY1 protein (arrow).)

Western Blot (WB)

(CHSY1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the CHSY1 antibody detected the CHSY1 protein (arrow).)

Flow Cytometry (FC/FACS)

(CHSY1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CHSY1 antibody

CHSY1 synthesizes chondroitin sulfate, a glycosaminoglycan expressed on the surface of most cells and in extracellular matrices. Glycosaminoglycan chains are covalently linked to a wide range of core protein families and regulate many biologic processes, including cell proliferation and recognition, extracellular matrix deposition, and morphogenesis.[supplied by OMIM].

Product Categories/Family for anti-CHSY1 antibody

Antibodies; Enzymes; Glucuronosyltransferase

References

Yada, T., et al. J. Biol. Chem. 278(41):39711-39725(2003). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Kitagawa, H., et al. J. Biol. Chem. 278(26):23666-23671(2003). Kitagawa, H., et al. J. Biol. Chem. 276(42):38721-38726(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 31542309
• NCBI GeneID: 22856
• NCBI Accession #: NP_055733.2
• NCBI GenBank Nucleotide #: NM_014918.4
• UniProt Accession #: Q86X52; Q6UX38; Q7LFU5; Q9Y2J5
• Molecular Weight: 91,784 Da
• NCBI Official Full Name: chondroitin sulfate synthase 1
• NCBI Official Synonym Full Names: chondroitin sulfate synthase 1
• NCBI Official Symbol: CHSY1
• NCBI Official Synonym Symbols: CHSY; CSS1; TPBS; ChSy-1
• NCBI Protein Information: chondroitin sulfate synthase 1; chondroitin synthase 1; carbohydrate synthase 1; chondroitin glucuronyltransferase 1; N-acetylgalactosaminyltransferase II; chondroitin glucuronyltransferase II; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransf
• UniProt Protein Name: Chondroitin sulfate synthase 1
• Protein Family: Chondroitin sulfate synthase
• UniProt Gene Name: CHSY1
• UniProt Synonym Gene Names: CHSY; CSS1; KIAA0990; ChSy-1
• UniProt Entry Name: CHSS1_HUMAN

NCBI Description

This gene encodes a member of the chondroitin N-acetylgalactosaminyltransferase family. These enzymes possess dual glucuronyltransferase and galactosaminyltransferase activity and play critical roles in the biosynthesis of chondroitin sulfate, a glycosaminoglycan involved in many biological processes including cell proliferation and morphogenesis. Decreased expression of this gene may play a role in colorectal cancer, and mutations in this gene are a cause of temtamy preaxial brachydactyly syndrome. [provided by RefSeq, Dec 2011]

Uniprot Description

CHSY1: Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Involved in the negative control of osteogenesis likely through the modulation of NOTCH signaling. Defects in CHSY1 are the cause of Temtamy preaxial brachydactyly syndrome (TPBS). A syndrome characterized by multiple congenital anomalies, mental retardation, sensorineural deafness, talon cusps of upper central incisors, growth retardation, and bilateral symmetric digital anomalies mainly in the form of preaxial brachydactyly and hyperphalangism. Belongs to the chondroitin N- acetylgalactosaminyltransferase family.

Protein type: EC 2.4.1.175; EC 2.4.1.226; Transferase; Membrane protein, integral; Glycan Metabolism - chondroitin sulfate biosynthesis

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: Golgi membrane; membrane; integral to membrane; extracellular region

Molecular Function: N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity; metal ion binding; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity

Biological Process: response to nutrient levels; chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; sulfation; negative regulation of ossification; positive regulation of smoothened signaling pathway; pathogenesis; chondrocyte development; proximal/distal pattern formation

Disease: Temtamy Preaxial Brachydactyly Syndrome