Rabbit NFkappaB-p65 Polyclonal Antibody | anti-NFkappaB-p65 antibody

Anti-NFkappaB-p65 (Phospho-Ser536) Rabbit pAb

Cat. Num. AAA476977

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Synonyms: NFkappaB-p65; Polyclonal Antibody; Anti-NFkappaB-p65 (Phospho-Ser536) Rabbit pAb; NFKB3; RELA; TF65; Transcription factor p65; p65; anti-NFkappaB-p65 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/mL (varies by lot)
• Sequence Length: 548

• Applicable Applications for anti-NFkappaB-p65 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:100-1:200; IHC: 1:50-1:100
• Immunogen: Peptide sequence around phosphorylation site of serine 536 (F-S-S (p)-I-A) derived from Human NFkappaB-p65.
• Sensitivity: The antibody detects endogenous level of NF-kappaB p65 only when phosphorylated at serine 536.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 and HL60 cells untreated or treated with TNF using NFkappaB-p65 (Phospho-Ser536) Antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using NFkappaB-p65 (Phospho-Ser536) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkappaB-p65 (Phospho-Ser536) Antibody (left) or the same antibody preincubated with blocking peptide #51014 (right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed MEF cells using NFkappaB-p65 (Phospho-Ser536) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkappaB-p65 (Phospho-Ser536) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using NFkappaB-p65 (Phospho-Ser536) Antibody.)

Related Product Information for anti-NFkappaB-p65 antibody

Entrez Summary: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 65
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: RELA proto-oncogene, NF-kB subunit
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway