Western Blot (WB)
(CHST5 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CHST5 antibody detected the CHST5 protein (arrow).)
Rabbit anti-Human CHST5 Polyclonal Antibody | anti-CHST5 antibody
CHST5 Antibody (C-term)
Western Blot (WB)
(CHST5 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CHST5 antibody detected the CHST5 protein (arrow).)
Western Blot (WB)
(CHST5 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CHST5 antibody detected the CHST5 protein (arrow).)
structures with variation in monosaccharide composition, glycosidic
linkage positions, and branching of chains. Further diversity is
added by the covalent addition of sulfate moieties to particular
hydroxyl groups and amino groups of saccharides. The sulfate
modifications of glycoproteins can be extensive in amount and
frequently occur at high density. They can have a profound effect
on the physiochemical properties of the glycoconjugates, at least
in part through the addition of negative charge. Carbohydrate
sulfation plays a critical role in many biologic processes. CHST5
belongs to the GST family of sulfotransferases, which also includes
CHST1 (MIM 603797), CHST2 (MIM 603798), CHST3 (MIM 603799), and
LSST. These enzymes are 6-O-sulfotransferases, which add sulfate to
C6 of galactose (Gal), N-acetylgalactosamine (GalNAc), or
N-acetylglucosamine (GlcNAc) (Lee et al., 1999 [PubMed
10491328]).
Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
Kitayama, K., et al. J. Biol. Chem. 282(41):30085-30096(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family, members of which catalyze the transfer of sulfate to position 6 of galactose (Gal), N-acetylgalactosamine (GalNAc), or N-acetylglucosamine (GlcNAc) residues within proteoglycans, and sulfation of O-linked sugars of mucin-type acceptors. Carbohydrate sulfation plays a critical role in many biologic processes. This gene is predominantly expressed in colon and small intestine. [provided by RefSeq, Aug 2011]
Uniprot Description
CHST5: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; Membrane protein, integral; EC 2.8.2.-
Chromosomal Location of Human Ortholog: 16q22.3
Cellular Component: Golgi membrane; Golgi apparatus; intrinsic to Golgi membrane; integral to membrane
Molecular Function: sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity
Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; protein amino acid sulfation; keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; carbohydrate metabolic process; pathogenesis
Research Articles on CHST5
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Product Notes
The CHST5 chst5 (Catalog #AAA9201961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 275-302. The CHST5 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHST5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CHST5 chst5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHST5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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