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Western Blot (WB) (Western blot analysis of CHST14 using HepG2 whole cell lysates)

Rabbit CHST14 Polyclonal Antibody | anti-CHST14 antibody

CHST14 Antibody

Gene Names
CHST14; ATCS; D4ST1; EDSMC1; HNK1ST
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CHST14; Polyclonal Antibody; CHST14 Antibody; 2600016L03Rik; ATCS; Carbohydrate (N acetylgalactosamine 4 0) sulfotransferase 14; Carbohydrate sulfotransferase 14; CHSTE_HUMAN; D4ST-1; D4st1; Dermatan 4-sulfotransferase 1; hD4ST1; RP23-286G12.2; UNQ1925/PRO4400; anti-CHST14 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CHST14 antibody detects endogenous levels of total CHST14
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
376
Applicable Applications for anti-CHST14 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human CHST14
Subcellular Location
Golgi Apparatus Membrane.
Tissue Specificity
Widely expressed. Expressed at high level in pituitary gland, placenta, uterus and thyroid.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Sheep, Dog
Similarity
Pig (100%), Zebrafish (100%), Bovine (91%), Sheep (91%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CHST14 using HepG2 whole cell lysates)

Western Blot (WB) (Western blot analysis of CHST14 using HepG2 whole cell lysates)

Western Blot (WB)

(Western blot analysis of extracts from rat muscle and rat brain, using CHST14 Antibody.)

Western Blot (WB) (Western blot analysis of extracts from rat muscle and rat brain, using CHST14 Antibody.)
Related Product Information for anti-CHST14 antibody
Function: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1, 4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in-IdoUA-GalNAc-IdoUA-than in-GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. Appears to have an important role in the formation of the cerebellar neural network during postnatal brain development.
Similarity: Belongs to the sulfotransferase 2 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 43 kDa
Predicted: 43 kDa
NCBI Official Full Name
carbohydrate sulfotransferase 14
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 14
NCBI Official Symbol
CHST14
NCBI Official Synonym Symbols
ATCS; D4ST1; EDSMC1; HNK1ST
NCBI Protein Information
carbohydrate sulfotransferase 14
UniProt Protein Name
Carbohydrate sulfotransferase 14
UniProt Gene Name
CHST14
UniProt Synonym Gene Names
D4ST1; D4ST-1; hD4ST1

NCBI Description

This gene encodes a member of the HNK-1 family of sulfotransferases. The encoded protein transfers sulfate to the C-4 hydroxyl of N-acetylgalactosamine residues in dermatan sulfate. Mutations in this gene have been associated with adducted thumb-clubfoot syndrome.[provided by RefSeq, Mar 2010]

Uniprot Description

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. Appears to have an important role in the formation of the cerebellar neural network during postnatal brain development.

Research Articles on CHST14

Similar Products

Product Notes

The CHST14 chst14 (Catalog #AAA9609648) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHST14 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHST14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the CHST14 chst14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHST14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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