Rabbit CHST14 Polyclonal Antibody | anti-CHST14 antibody

CHST14 Rabbit Polyclonal

Cat. Num. AAA767926

• Gene Names: CHST14; ATCS; D4ST1; EDSMC1; HNK1ST
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: CHST14; Polyclonal Antibody; CHST14 Rabbit Polyclonal; anti-CHST14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 376

• Applicable Applications for anti-CHST14 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta using MBS767926 (CHST14 antibody) at dilution of 1:50)

Western Blot (WB)

(HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS767926 (CHST14 antibody) at dilution of 1:600)

NCBI and Uniprot Product Information

• NCBI GI #: 18497304
• NCBI GeneID: 113189
• NCBI Accession #: NP_569735.1
• NCBI GenBank Nucleotide #: NM_130468.3
• Molecular Weight: 42,997 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 14
• NCBI Official Synonym Full Names: carbohydrate sulfotransferase 14
• NCBI Official Symbol: CHST14
• NCBI Official Synonym Symbols: ATCS; D4ST1; EDSMC1; HNK1ST
• NCBI Protein Information: carbohydrate sulfotransferase 14
• UniProt Protein Name: Carbohydrate sulfotransferase 14
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST14
• UniProt Synonym Gene Names: D4ST1; D4ST-1; hD4ST1
• UniProt Entry Name: CHSTE_HUMAN

NCBI Description

This gene encodes a member of the HNK-1 family of sulfotransferases. The encoded protein transfers sulfate to the C-4 hydroxyl of N-acetylgalactosamine residues in dermatan sulfate. Mutations in this gene have been associated with adducted thumb-clubfoot syndrome.[provided by RefSeq, Mar 2010]

Uniprot Description

CHST14: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. GlcUA to IdoUA. Appears to have an important role in the formation of the cerbellar neural network during postnatal brain development. Defects in CHST14 are the cause of Ehlers-Danlos syndrome musculocontractural type (EDSMC). It is a form of Ehlers-Danlos syndrome characterized by distinctive craniofacial dysmorphism, congenital contractures of thumbs and fingers, clubfeet, severe kyphoscoliosis, muscular hypotonia, hyperextensible thin skin with easy bruisability and atrophic scarring, wrinkled palms, joint hypermobility, and ocular involvement. Belongs to the sulfotransferase 2 family.

Protein type: Transferase; Membrane protein, integral; EC 2.8.2.35; Glycan Metabolism - chondroitin sulfate biosynthesis

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: N-acetylgalactosamine 4-O-sulfotransferase activity; phosphate binding

Biological Process: carbohydrate biosynthetic process; dermatan sulfate biosynthetic process; dermatan sulfate proteoglycan metabolic process

Disease: Ehlers-danlos Syndrome, Musculocontractural Type 1