Western Blot (WB)
(Western blot analysis of CHRNA4 using HuvEc whole cell lysates)
Rabbit anti-Human CHRNA4 Polyclonal Antibody | anti-CHRNA4 antibody
CHRNA4 Antibody
Gene Names
CHRNA4; EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CHRNA4; Polyclonal Antibody; CHRNA4 Antibody; A4 nicotinic receptor; Acetylcholine receptor alpha 4 neural; Acetylcholine receptor neuronal nicotinic alpha 4 subunit; ACH 4; ACH4; ACHA4_HUMAN; AChR; Acra 4; Acra4; Alpha4 nAChR; BFNC; Cholinergic receptor nicotinic alpha 4; Cholinergic receptor nicotinic alpha polypeptide 4; CHRNA 4; EBN 1; EBN; EBN1; NACHR; NACRA 4; NACRA4; Neuronal acetylcholine receptor subunit alpha-4; Neuronal nicotinic acetylcholine receptor alpha 4 subunit; anti-CHRNA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CHRNA4 antibody detects endogenous levels of total CHRNA4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
627
Applicable Applications for anti-CHRNA4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human CHRNA4
Subcellular Location
Cell Junction > Synapse > Postsynaptic Cell Membrane. Cell Membrane.
Predicted Cross Reactivity
Pig, Bovine, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CHRNA4 using HuvEc whole cell lysates)
Western Blot (WB)
(Western blot analysis of CHRNA4 using HuvEc whole cell lysates)
Related Product Information for anti-CHRNA4 antibody
Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
Subunit Structure: Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers (PubMed:22361591). Interacts with RIC3; which is required for proper folding and assembly (PubMed:16120769). Interacts with LYPD6 (PubMed:27344019). The heteropentamer alpha-4-beta-2 interacts with alpha-conotoxins PnIA, GID and MII (By similarity).
Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
Subunit Structure: Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers (PubMed:22361591). Interacts with RIC3; which is required for proper folding and assembly (PubMed:16120769). Interacts with LYPD6 (PubMed:27344019). The heteropentamer alpha-4-beta-2 interacts with alpha-conotoxins PnIA, GID and MII (By similarity).
Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 70 kDa
Predicted: 70 kDa
Predicted: 70 kDa
NCBI Official Full Name
neuronal acetylcholine receptor subunit alpha-4 isoform 1
NCBI Official Synonym Full Names
cholinergic receptor nicotinic alpha 4 subunit
NCBI Official Symbol
CHRNA4
NCBI Official Synonym Symbols
EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4
NCBI Protein Information
neuronal acetylcholine receptor subunit alpha-4
UniProt Protein Name
Neuronal acetylcholine receptor subunit alpha-4
Protein Family
UniProt Gene Name
CHRNA4
UniProt Synonym Gene Names
NACRA4
NCBI Description
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Uniprot Description
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
Research Articles on CHRNA4
Similar Products
Product Notes
The CHRNA4 chrna4 (Catalog #AAA9608618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHRNA4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHRNA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the CHRNA4 chrna4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHRNA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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