Rabbit CHRM2 Polyclonal Antibody | anti-CHRM2 antibody

Anti-CHRM2 Antibody

Cat. Num. AAA178669

• Gene Names: CHRM2; HM2
• Reactivity: Reacts with: Mouse, Rat; Predicted to work with: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: CHRM2; Polyclonal Antibody; Anti-CHRM2 Antibody; Acm2; ACM2; CHRM 2; M2 muscarinic receptor; Muscarinic Acetylcholine Receptor 2; Muscarinic M2 receptor; P08172; Muscarinic acetylcholine receptor M2; cholinergic receptor; muscarinic 2; anti-CHRM2 antibody

• Host: Rabbit
• Reactivity: Reacts with: Mouse, Rat; Predicted to work with: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCI , 0.2mg Na₂HPO₄, 0.05mg NaN₃
• Sequence Length: 466

• Applicable Applications for anti-CHRM2 antibody: Western Blot (WB)
• Application Notes: Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Mouse , Rat; Predicted Species: Human; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CHRM2 (352-376aa QNGDEKQNIVARKIVKMTKQPAKKK), identical to the related mouse and rat sequences.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of CHRM2 expression in rat brain extract (lane 1) and mouse brain extract (lane 2). CHRM2 at 52KD was detected using rabbit anti- CHRM2 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-CHRM2 antibody

Background: The muscarinic acetylcholine receptor M2, also known as the cholinergic receptor, muscarinic 2, is a muscarinic acetylcholine receptor that in humans is encoded by the CHRM2 gene. The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.

References

1. "Entrez Gene: CHRM2 cholinergic receptor, muscarinic 2".
2. Comings DE, Wu S, Rostamkhani M, McGue M, Lacono WG, Cheng LS, MacMurray JP (January 2003). "Role of the cholinergic muscarinic 2 receptor (CHRM2) gene in cognition". Mol. Psychiatry. 8 (1): 10-1.
3. Gosso MF, van Belzen M, de Geus EJ, Polderman JC, Heutink P, Boomsma DI, Posthuma D (November 2006). "Association between the CHRM2 gene and intelligence in a sample of 304 Dutch families". Genes Brain Behav. 5(8): 577-84.

NCBI and Uniprot Product Information

• NCBI GI #: 4502817
• NCBI GeneID: 1129
• NCBI Accession #: NP_000730.1
• NCBI GenBank Nucleotide #: NM_000739.2
• UniProt Accession #: P08172; Q4VBK6; Q9P1X9
• NCBI Official Full Name: muscarinic acetylcholine receptor M2
• NCBI Official Synonym Full Names: cholinergic receptor muscarinic 2
• NCBI Official Symbol: CHRM2
• NCBI Official Synonym Symbols: HM2
• NCBI Protein Information: muscarinic acetylcholine receptor M2
• UniProt Protein Name: Muscarinic acetylcholine receptor M2
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM2

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR M2: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD). MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: integral to plasma membrane; plasma membrane; synapse

Molecular Function: G-protein coupled acetylcholine receptor activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; nervous system development; regulation of smooth muscle contraction; response to virus; synaptic transmission, cholinergic