Rabbit CHP2 Polyclonal Antibody | anti-CHP2 antibody

CHP2 Polyclonal Antibody

Cat. Num. AAA3005758

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CHP2; Polyclonal Antibody; CHP2 Polyclonal Antibody; Calcineurin B homologous protein 2; Hepatocellular carcinoma-associated antigen 520; HCA520; anti-CHP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHP2 polyclonal antibody detects endogenous levels of CHP2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 196

• Applicable Applications for anti-CHP2 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 146-191 of Human CHP2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CHP2 polyclonal antibody at 1:500 dilutionLane1:HepG2 cell lysateLane2:Mouse liver tissue lysateLane3:H9C2 cell lysate)

Related Product Information for anti-CHP2 antibody

CHP2 (Calcineurin B homologous protein 2), also known as Hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains four EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 11545811
• NCBI GeneID: 63928
• NCBI Accession #: NP_071380.1
• NCBI GenBank Nucleotide #: NP_071380.1
• UniProt Accession #: O43745
• Molecular Weight: ~ 22kDa
• NCBI Official Full Name: calcineurin B homologous protein 2
• NCBI Official Synonym Full Names: calcineurin like EF-hand protein 2
• NCBI Official Symbol: CHP2
• NCBI Protein Information: calcineurin B homologous protein 2
• UniProt Protein Name: Calcineurin B homologous protein 2
• Protein Family: Calcineurin B homologous protein
• UniProt Gene Name: CHP2
• UniProt Synonym Gene Names: HCA520
• UniProt Entry Name: CHP2_HUMAN

NCBI Description

This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Another member of the CHP subfamily, Calcineurin B homologous protein 1, is located on Chromosome 15 and is an inhibitor of calcineurin activity and has a genetic phenotype associated with Parkinson's Disease (OMIM:606988). This gene was initially identified as a tumor-associated antigen and was previously referred to as Hepatocellular carcinoma-associated antigen 520. [provided by RefSeq, Jul 2013]

Uniprot Description

CHP2: Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation- induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus. Belongs to the calcineurin regulatory subunit family. CHP subfamily.

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; calcium ion binding

Biological Process: protein transport; positive regulation of protein import into nucleus; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter