Rabbit Cholera Toxin, beta Polyclonal Antibody
Cholera Toxin, beta (HRP)
Applications
ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Gel Super Shift Assay
Purity
Highly PurifiedPurified IgG fraction to a purity of ~95%.
Synonyms
Cholera Toxin; beta; Polyclonal Antibody; beta (HRP); Anti -Cholera Toxin; anti-Cholera Toxin; beta antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
Purity/Purification
Highly Purified
Purified IgG fraction to a purity of ~95%.
Purified IgG fraction to a purity of ~95%.
Form/Format
Supplied as a liquid in PBS, 10mg/ml BSA, 0.002% thimerosal. Labeled with HRP. Estimated molar HRP
Applicable Applications for anti-Cholera Toxin, beta antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA, Western Blot, Immunocytochemistry, and Immunoprecipitation (Gel).
Dilution: Immunocytochemistry: 1:20-1:200
Western Blot: 1:20-1:200
ELISA: 1:200-1:000
Dilution: Immunocytochemistry: 1:20-1:200
Western Blot: 1:20-1:200
ELISA: 1:200-1:000
Immunogen
Purified Choleragenoid.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Cholera Toxin, beta antibody
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Product Categories/Family for anti-Cholera Toxin, beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
cholera toxin
Uniprot Description
ctxB: The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself
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Product Notes
The Cholera Toxin, beta (Catalog #AAA6008426) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cholera Toxin, beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA, Western Blot, Immunocytochemistry, and Immunoprecipitation (Gel). Dilution: Immunocytochemistry: 1:20-1:200 Western Blot: 1:20-1:200 ELISA: 1:200-1:000. Researchers should empirically determine the suitability of the Cholera Toxin, beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cholera Toxin, beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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