anti-Human APOBEC3A Antibody | anti-APOBEC3A antibody

APOBEC3A Antibody

Cat. Num. AAA8509907

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: APOBEC3A; APOBEC3A Antibody; EC 3.5.4.-; Phorbolin-1; anti-APOBEC3A antibody

• Reactivity: Human
• Specificity: APOBEC3A antibody detects endogenous levels of total APOBEC3A protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 199

• Applicable Applications for anti-APOBEC3A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human APOBEC3A.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using APOBEC3A antibody. For Research Use Only)

References

Madsen P.P., J. Invest. Dermatol. 113:162-169(1999). Collins J.E., Genome Biol. 5:R84.1-R84.11(2004). Dunham I., Nature 402:489-495(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 300869638
• NCBI GeneID: 100913187
• NCBI Accession #: NP_001180218.1
• NCBI GenBank Nucleotide #: NM_001193289.1
• UniProt Accession #: P31941; Q12807; Q5JZ93; Q9UH18; A0AVM1
• Molecular Weight: 21,778 Da
• NCBI Official Full Name: probable DNA dC->dU-editing enzyme APOBEC-3A
• NCBI Official Synonym Full Names: APOBEC3A and APOBEC3B deletion hybrid
• NCBI Official Symbol: APOBEC3A_B
• NCBI Protein Information: probable DNA dC->dU-editing enzyme APOBEC-3A
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3A
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3A
• UniProt Synonym Gene Names: A3A

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. The protein encoded by this gene is the same as that encoded by APOBEC3A; however, this gene is a hybrid gene that results from the deletion of approximately 29.5 kb of sequence between the APOBEC3A gene and the adjacent gene APOBEC3B. The breakpoints of the deletion are within the two genes, so the deletion hybrid is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. [provided by RefSeq, Jul 2012]

Uniprot Description

DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.