Rabbit CHMP5 Polyclonal Antibody | anti-CHMP5 antibody

CHMP5 Antibody

Cat. Num. AAA9630122

• Gene Names: CHMP5; Vps60; CGI-34; PNAS-2; C9orf83; HSPC177; SNF7DC2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CHMP5; Polyclonal Antibody; CHMP5 Antibody; apoptosis-related protein PNAS-2; C9orf83; CGI 34; Charged multivesicular body protein 5; CHMP 5; CHMP family; member 5; chmp5; CHMP5_HUMAN; Chromatin modifying protein 5; Chromatin-modifying protein 5; Chromosome 9 open reading frame 83; HGNC:26942; HSPC177; hVps60; PNAS 2; SNF7 domain containing protein 2; SNF7 domain-containing protein 2; SNF7DC2; Vacuolar protein sorting 60; Vacuolar protein sorting-associated protein 60; Vps60; anti-CHMP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CHMP5 Antibody detects endogenous levels of CHMP5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CHMP5 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human CHMP5.
• Conjugation: Unconjugated
• Similarity: Belongs to the SNF7 family.
• Post Translational Modifications: ISGylated. Isgylation inhibits its interaction with VTA1.
• Subunit Structure: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Interacts with VTA1. Interacts with CHMP2A. Interacts with VTA1; the interaction involves soluble CHMP5. Interacts with NOD2.
• Function: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I, -II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
• Note: Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CHMP5 antibody

Cytoplasm; Cytosol; Endosome membrane; Peripheral membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 306966146
• NCBI GeneID: 51510
• NCBI Accession #: NP_001182465.1
• NCBI GenBank Nucleotide #: NM_001195536.1
• UniProt Accession #: Q9NZZ3; Q5VXW2; Q96AV2; Q9HB68; Q9NYS4; Q9Y323; B2RD95; B4DIR6
• Molecular Weight: 19,520 Da
• NCBI Official Full Name: charged multivesicular body protein 5 isoform 2
• NCBI Official Synonym Full Names: charged multivesicular body protein 5
• NCBI Official Symbol: CHMP5
• NCBI Official Synonym Symbols: Vps60; CGI-34; PNAS-2; C9orf83; HSPC177; SNF7DC2
• NCBI Protein Information: charged multivesicular body protein 5; SNF7 domain containing 2; SNF7 domain-containing protein 2; apoptosis-related protein PNAS-2; chromatin modifying protein 5; chromatin-modifying protein 5; hVps60; vacuolar protein sorting-associated protein 60
• UniProt Protein Name: Charged multivesicular body protein 5
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP5
• UniProt Synonym Gene Names: C9orf83; SNF7DC2; Vps60; hVps60
• UniProt Entry Name: CHMP5_HUMAN

NCBI Description

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

Uniprot Description

CHMP5: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: endosome membrane; nucleus; cytosol

Molecular Function: protein binding

Biological Process: protein transport; viral reproduction; cell separation during cytokinesis; lysosome organization and biogenesis; regulation of receptor recycling; endosome to lysosome transport; viral infectious cycle; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis