Rabbit CHMP4C Polyclonal Antibody | anti-CHMP4C antibody

CHMP4C Antibody

Cat. Num. AAA9630653

• Gene Names: CHMP4C; Shax3; SNF7-3; VPS32C
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CHMP4C; Polyclonal Antibody; CHMP4C Antibody; Charged multivesicular body protein 4c; CHM4C_HUMAN; CHMP4c; Chromatin-modifying protein 4c; hSnf7-3; hVps32-3; SHAX3; SNF7 homolog associated with Alix 3; SNF7-3; Vacuolar protein sorting-associated protein 32-3; Vps32-3; anti-CHMP4C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CHMP4C Antibody detects endogenous levels of CHMP4C.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CHMP4C antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human CHMP4C.
• Conjugation: Unconjugated
• Similarity: The acidic C-terminus and the basic N-terminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components (By similarity). Belongs to the SNF7 family.
• Post Translational Modifications: Phosphorylated at Ser-210 by AURKB during cytokinesis: together with ZFYVE19/ANCHR, phosphorylated CHMP4C retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis.
• Subunit Structure: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Self-associates. Interacts with CHMP2A. Interacts with CHMP4A. Interacts with CHMP4B. Interacts with CHMP6. Interacts with VPS4A. Interacts with PDCD6IP; the interaction is direct.
• Tissue Specificity: Expressed in heart, spleen and kidney.
• Function: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I, -II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: upon phosphorylation by AURKB, together with ZFYVE19/ANCHR, retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ANCHR and VPS4 and subsequent abscission. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan.
• Note: Localizes to the midbody during late cytokinesis. During its recruitment, localizes initially to the midbody arms, before being directed to the central region, the midbody ring, also called Flemming body. Phosphorylation at Ser-210 by AURKB triggers localization to midbody ring.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CHMP4C antibody

Cytoplasm; Cytosol; Late endosome membrane; Peripheral membrane protein; Midbody; Midbody ring; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 22748643
• NCBI GeneID: 92421
• NCBI Accession #: NP_689497.1
• NCBI GenBank Nucleotide #: NM_152284.3
• UniProt Accession #: Q96CF2; B2RBZ1
• Molecular Weight: 26,411 Da
• NCBI Official Full Name: charged multivesicular body protein 4c
• NCBI Official Synonym Full Names: charged multivesicular body protein 4C
• NCBI Official Symbol: CHMP4C
• NCBI Official Synonym Symbols: Shax3; SNF7-3; VPS32C
• NCBI Protein Information: charged multivesicular body protein 4c; hSnf7-3; vps32-3; hVps32-3; chromatin modifying protein 4C; chromatin-modifying protein 4c; SNF7 homolog associated with Alix 3; Snf7 homologue associated with Alix 3; vacuolar protein sorting-associated protein 32-
• UniProt Protein Name: Charged multivesicular body protein 4c
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP4C
• UniProt Synonym Gene Names: SHAX3; CHMP4c; hSnf7-3; Vps32-3; hVps32-3
• UniProt Entry Name: CHM4C_HUMAN