Western Blot (WB)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Hamartin/TSC1 Polyclonal Antibody | anti-TSC1 antibody
Goat anti-Hamartin/TSC1 (isoform 1) Antibody
Immunohistochemistry: In paraffin embedded Human Uterus shows membranous staining around non-basal nuclei and at the lumen of the endometrial gland. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet.2000 Jul 22;9(12): 1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx.120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing Human TSC1 (RC213332) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing Human TSC1 (RC213332) and probed (mock transfection in first lane), tested by Origene.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
Uniprot Description
TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.
Protein type: Motility/polarity/chemotaxis; Tumor suppressor
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: actin filament; cell cortex; cytoplasm; cytosol; growth cone; lamellipodium; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; TSC1-TSC2 complex
Molecular Function: chaperone binding; GTPase activating protein binding; GTPase regulator activity; protein binding; protein N-terminus binding
Biological Process: adaptive immune response; adult locomotory behavior; cardiac muscle cell differentiation; cell cycle arrest; cell projection organization and biogenesis; cell-matrix adhesion; cerebral cortex development; glucose import; hippocampus development; kidney development; memory T cell differentiation; myelination; negative regulation of cell proliferation; negative regulation of cell size; negative regulation of insulin receptor signaling pathway; negative regulation of macroautophagy; negative regulation of TOR signaling pathway; negative regulation of translation; neural tube closure; positive regulation of focal adhesion formation; potassium ion transport; protein heterooligomerization; protein stabilization; regulation of cell cycle; regulation of cell-matrix adhesion; regulation of phosphoprotein phosphatase activity; regulation of protein kinase activity; regulation of stress fiber formation; regulation of translation; response to insulin stimulus; rRNA export from nucleus; synapse organization and biogenesis
Disease: Focal Cortical Dysplasia Of Taylor; Lymphangioleiomyomatosis; Tuberous Sclerosis 1
Research Articles on TSC1
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Product Notes
The TSC1 tsc1 (Catalog #AAA420710) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Hamartin/TSC1 (isoform 1) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Hamartin/TSC1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Uterus shows membranous staining around non-basal nuclei and at the lumen of the endometrial gland. Recommended concentration, 3-6ug/ml. Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet.2000 Jul 22;9(12): 1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx.120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the TSC1 tsc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QLHIMDYNET HHEH. It is sometimes possible for the material contained within the vial of "Hamartin/TSC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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