Rabbit CHK2 Polyclonal Antibody | anti-CHK2 antibody

CHK2 Antibody (Thr387)

Cat. Num. AAA5313773

• Gene Names: CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: CHK2; Polyclonal Antibody; CHK2 Antibody (Thr387); Rabbit Polyclonal CHK2 Antibody (Thr387); Polyclonal CHK2 antibody; Anti-CHK2 antibody; Cds1 antibody; checkpoint-like protein CHK2 antibody; CHEK2 antibody; CHK2 antibody; CHK2 checkpoint homolog (S. pombe) antibody; HuCds1 antibody; LFS2 antibody; PP1425 antibody; protein kinase CHK2 antibody; RAD53 antibody; Serine/threonine-protein kinase Chk2 antibody; anti-CHK2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHK2 antibody (Thr387) detects endogenous levels of CHK2 only when phosphorylated at serine 317
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 514

• Applicable Applications for anti-CHK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100; ELISA: 1:4000
• Immunogen: CHK2 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human CHK2 around the phosphorylation site of threonine 387 (C-G-TP-P-T) as the immunogen.
• Cross-Reactivity: Mouse, Rat
• Preparation and Storage: Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK2 antibody (Thr387))

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells using CHK2 (Phospho-Thr387) antibody)

Related Product Information for anti-CHK2 antibody

CHK2 phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest.

Product Categories/Family for anti-CHK2 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 11034732
• NCBI GeneID: 11200
• NCBI Official Full Name: CHK2, partial
• NCBI Official Synonym Full Names: checkpoint kinase 2
• NCBI Official Symbol: CHEK2
• NCBI Official Synonym Symbols: CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• NCBI Protein Information: serine/threonine-protein kinase Chk2

NCBI Description

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]