Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CGRRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit anti-Human CGRRF1 Polyclonal Antibody | anti-CGRRF1 antibody
CGRRF1 Rabbit pAb
Gene Names
CGRRF1; CGR19; RNF197
Reactivity
Human
Applications
Western Blot
Purity
Affinity purification
Synonyms
CGRRF1; Polyclonal Antibody; CGRRF1 Rabbit pAb; CGR19; RNF197; anti-CGRRF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
VPVILRNSEETQFSTRVFKKQMRQVKNPFGLEITNPSSASITTGITLTTDCLEDSLLTCYWGCSVQKLYEALQKHVYCFRISTPQALEDALYSEYLYQEQYFIKKDSKEEIYCQLPRDTKIEDFGTVPRSR
Applicable Applications for anti-CGRRF1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-170 of human CGRRF1 (NP_006559.1).
Positive Samples
HepG2, 293T
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CGRRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CGRRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Product Categories/Family for anti-CGRRF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,242 Da
NCBI Official Full Name
cell growth regulator with RING finger domain protein 1
NCBI Official Synonym Full Names
cell growth regulator with ring finger domain 1
NCBI Official Symbol
CGRRF1
NCBI Official Synonym Symbols
CGR19; RNF197
NCBI Protein Information
cell growth regulator with RING finger domain protein 1
UniProt Protein Name
Cell growth regulator with RING finger domain protein 1
Protein Family
UniProt Gene Name
CGRRF1
UniProt Synonym Gene Names
CGR19; RNF197
UniProt Entry Name
CGRF1_HUMAN
Uniprot Description
Function: Able to inhibit growth in several cell lines
By similarity.
Subcellular location: Nucleus Ref.3.
Sequence similarities: Contains 1 RING-type zinc finger.
Research Articles on CGRRF1
Similar Products
Product Notes
The CGRRF1 cgrrf1 (Catalog #AAA9142884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CGRRF1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CGRRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CGRRF1 cgrrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VPVILRNSEE TQFSTRVFKK QMRQVKNPFG LEITNPSSAS ITTGITLTTD CLEDSLLTCY WGCSVQKLYE ALQKHVYCFR ISTPQALEDA LYSEYLYQEQ YFIKKDSKEE IYCQLPRDTK IEDFGTVPRS R. It is sometimes possible for the material contained within the vial of "CGRRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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