Rabbit anti-Human CF150 Polyclonal Antibody | anti-CF150 antibody

CF150, ID (MB21D1, C6orf150, Protein MB21D1, Mab-21 domain-containing protein 1) (FITC)

Cat. Num. AAA6285318

• Gene Names: MB21D1; cGAS; h-cGAS; C6orf150
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CF150; Polyclonal Antibody; ID (MB21D1; C6orf150; Protein MB21D1; Mab-21 domain-containing protein 1) (FITC); MB21D1; Mab-21 domain-containing protein 1; anti-CF150 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CF150.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CF150 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 266-295 in the central region of human CF150.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MDA-MB231 cell line lysates (35ug/lane) usingMBS6001732.)

Western Blot (WB)

(Western Blot analysis of lysate from THP-1 cell line (20ug) using MBS6001732 (1:1000). IgG (HRP) goat anti-rabbit at 1:10,000 was used as the secondary antibody.)

Western Blot (WB)

(Western Blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate (from left to right, 20ug/lane), using MBS6001732 (1:1000/lane). IgG (HRP) goat anti-rabbit at 1:10,000 was used as the secondary antibody.)

Testing Data

(Overlay histogram showing U-2OS cells stained with MBS6001732 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6001732 (1:25) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events was performed.)

Product Categories/Family for anti-CF150 antibody

Antibodies; KIAA; orf

References

1. Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). 2. Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). 3. Mungall, A.J., et al. Nature 425(6960):805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 115511030
• NCBI GeneID: 115004
• NCBI Accession #: NP_612450.2
• NCBI GenBank Nucleotide #: NM_138441.2
• UniProt Accession #: Q8N884; Q14CV6; Q32NC9; Q5SWL0; Q5SWL1; Q96E45; L0L2J9
• Molecular Weight: 58,814 Da
• NCBI Official Full Name: cyclic GMP-AMP synthase
• NCBI Official Synonym Full Names: Mab-21 domain containing 1
• NCBI Official Symbol: MB21D1
• NCBI Official Synonym Symbols: cGAS; h-cGAS; C6orf150
• NCBI Protein Information: cyclic GMP-AMP synthase; cGAMP synthase; protein MB21D1; mab-21 domain-containing protein 1
• UniProt Protein Name: Cyclic GMP-AMP synthase
• UniProt Gene Name: MB21D1
• UniProt Synonym Gene Names: C6orf150; cGAMP synthase; cGAS; h-cGAS
• UniProt Entry Name: CGAS_HUMAN

Uniprot Description

MB21D1: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

Protein type: Enzyme, cellular metabolism; EC 2.7.7.86

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: cytosol

Molecular Function: ATP binding; DNA binding; GTP binding; metal ion binding

Biological Process: activation of innate immune response; cyclic nucleotide biosynthetic process; defense response to virus; innate immune response; positive regulation of defense response to virus by host; positive regulation of interferon type I production