Testing Data (TD)
(Anti-CEPT1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit CEPT1 Polyclonal Antibody | anti-CEPT1 antibody
CEPT1 Antibody (N-term)
Predicted Reactivity: Rat, Xenopus
Predicted Reactivity: Rat, Xenopus
IHC-P-Leica~~1:500
Testing Data (TD)
(Anti-CEPT1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(Anti-CEPT1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(All lanes : Anti-CEPT1 Antibody (N-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Human cerebellum tissue lysate Lane 4: SK-BR-3 whole cell lysate Lane 5: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(All lanes : Anti-CEPT1 Antibody (N-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Human cerebellum tissue lysate Lane 4: SK-BR-3 whole cell lysate Lane 5: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(All lanes : Anti-CEPT1 Antibody (N-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Human cerebellum tissue lysate Lane 3: SK-BR-3 whole cell lysate Lane 4: Human placenta tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(All lanes : Anti-CEPT1 Antibody (N-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Human cerebellum tissue lysate Lane 3: SK-BR-3 whole cell lysate Lane 4: Human placenta tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast tissue using AP10372a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast tissue using AP10372a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemical
(Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP10372a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemical
(Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP10372a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
synthesis of phosphatidylcholine by the transfer of phosphocholine
from CDP-choline to diacylglycerol. The synthesis of
phosphatidylethanolamine by ethanolaminephosphotransferase occurs
using an analogous reaction. This gene codes for a
choline/ethanolaminephosphotransferase. The protein can synthesize
either choline- or ethanolamine- containing phospholipids. Two
alternatively spliced transcripts encoding the same isoform have
been identified.
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Wright, M.M., et al. Lipids 37(7):663-672(2002)
Henneberry, A.L., et al. Biochem. J. 339 (PT 2), 291-298 (1999) :
NCBI and Uniprot Product Information
NCBI Description
Cholinephosphotransferase catalyses the final step in the synthesis of phosphatidylcholine by the transfer of phosphocholine from CDP-choline to diacylglycerol. The synthesis of phosphatidylethanolamine by ethanolaminephosphotransferase occurs using an analogous reaction. This gene codes for a choline/ethanolaminephosphotransferase. The protein can synthesize either choline- or ethanolamine- containing phospholipids. Two alternatively spliced transcripts encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
CEPT1: Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP- ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity. Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
Protein type: EC 2.7.8.1; Membrane protein, integral; EC 2.7.8.2; Endoplasmic reticulum; Transferase; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: endoplasmic reticulum membrane; nuclear membrane; integral to membrane
Molecular Function: diacylglycerol cholinephosphotransferase activity; ethanolaminephosphotransferase activity; metal ion binding
Biological Process: CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process
Research Articles on CEPT1
Similar Products
Product Notes
The CEPT1 cept1 (Catalog #AAA9202985) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 29-57. The CEPT1 Antibody (N-term) reacts with Human, Mouse Predicted Reactivity: Rat, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's CEPT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:2000 IHC-P-Leica~~1:500. Researchers should empirically determine the suitability of the CEPT1 cept1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEPT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
