Rabbit anti-Human CEPT1 Polyclonal Antibody | anti-CEPT1 antibody

CEPT1, NT (CEPT1, Choline/ethanolaminephosphotransferase 1) (Biotin)

Cat. Num. AAA6285218

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CEPT1; Polyclonal Antibody; NT (CEPT1; Choline/ethanolaminephosphotransferase 1) (Biotin); Choline/ethanolaminephosphotransferase 1; anti-CEPT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CEPT1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CEPT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 29-57 at the N-terminal region of human CEPT1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HL60 cell line lysate (35ug/lane using antibodyMBS648577.)

Immunohistochemistry (IHC)

(IHC staining of FFPE human breast tissue using antibodyMBS648577. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization.)

Related Product Information for anti-CEPT1 antibody

Cholinephosphotransferase catalyses the final step in the synthesis of phosphatidylcholine by the transfer of phosphocholine from CDP-choline to diacylglycerol. The synthesis of phosphatidylethanolamine by ethanolaminephosphotransferase occurs using an analogous reaction. This gene codes for a choline/ethanolaminephosphotransferase. The protein can synthesize either choline-or ethanolamine-containing phospholipids. Two alternatively spliced transcripts encoding the same isoform have been identified.

Product Categories/Family for anti-CEPT1 antibody

Antibodies; Enzymes

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Lamesch, P., et al. Genomics 89(3):307-315(2007). Wright, M.M., et al. Lipids 37(7):663-672(2002). Henneberry, A.L., et al. Biochem. J. 339 (PT 2), 291-298 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 56118223
• NCBI GeneID: 10390
• NCBI Accession #: NP_001007795.1; NP_006081.1
• NCBI GenBank Nucleotide #: NM_001007794.1
• UniProt Accession #: Q9Y6K0; Q69YJ9; Q9P0Y8
• Molecular Weight: 46,554 Da
• NCBI Official Full Name: choline/ethanolaminephosphotransferase 1
• NCBI Official Synonym Full Names: choline/ethanolamine phosphotransferase 1
• NCBI Official Symbol: CEPT1
• NCBI Protein Information: choline/ethanolaminephosphotransferase 1; hCEPT1
• UniProt Protein Name: Choline/ethanolaminephosphotransferase 1
• Protein Family: Choline/ethanolaminephosphotransferase
• UniProt Gene Name: CEPT1
• UniProt Synonym Gene Names: hCEPT1
• UniProt Entry Name: CEPT1_HUMAN

NCBI Description

Cholinephosphotransferase catalyses the final step in the synthesis of phosphatidylcholine by the transfer of phosphocholine from CDP-choline to diacylglycerol. The synthesis of phosphatidylethanolamine by ethanolaminephosphotransferase occurs using an analogous reaction. This gene codes for a choline/ethanolaminephosphotransferase. The protein can synthesize either choline- or ethanolamine- containing phospholipids. Two alternatively spliced transcripts encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity. Ref.1 Ref.6

Catalytic activity: CDP-ethanolamine + 1,2-diacylglycerol = CMP + a phosphatidylethanolamine. Ref.1CDP-choline + 1,2-diacylglycerol = CMP + a phosphatidylcholine. Ref.1

Cofactor: Magnesium or manganese. Ref.1

Pathway: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3.Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein Ref.8.

Tissue specificity: Ubiquitously expressed. Ref.1

Sequence similarities: Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Biophysicochemical propertiesKinetic parameters:KM=37 µM for CDP-choline Ref.1 Ref.7KM=101 µM for CDP-ethanolamineVmax=10.5 nmol/min/mg enzyme with CDP-choline as substrateVmax=4.35 nmol/min/mg enzyme with CDP-ethanolamine as substrate

Sequence caution: The sequence AAF61194.1 differs from that shown. Reason: Frameshift at position 276.