Rabbit anti-Human, Mouse CEP72 Polyclonal Antibody | anti-CEP72 antibody

CEP72 Rabbit pAb

Cat. Num. AAA4754609

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: CEP72; Polyclonal Antibody; CEP72 Rabbit pAb; anti-CEP72 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-CEP72 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Fusion protein of human CEP72
• Conjugation: Unconjugated
• Molecular Function: Centrosomes are the major microtubule-organizing centers of mammalian cells. They are composed of a centriole pair and surrounding microtubule-nucleating material termed pericentriolar material (PCM). Bipolar mitotic spindle assembly relies on two intertwined processes: centriole duplication and centrosome maturation. Failure to properly orchestrate centrosome duplication and maturation is subsequently linked to spindle defects, which can result in aneuploidy and promote cancer progression. CEP72 (centrosomal protein 72kDa) is a 647 amino acid protein that localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. Involved in the recruitment of key centrosomal proteins to the centrosome, CEP72 provides centrosomal microtubule-nucleation activity on the © Tubulin ring complexes and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. CEP72 exists as two alternatively spliced isoforms.
• Modification: Unmodified
• Cell Position: Cytoplasm, Cytoskeleton
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: TM4, NIH/3T3 and Jurkat cell lysates, Primary antibody: 124513(CEP72 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 124513(CEP72 Antibody) at dilution 1/40. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 124513(CEP72 Antibody) at dilution 1/40. (Original magnification: ×200))

Product Categories/Family for anti-CEP72 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 12803000
• NCBI GeneID: 55722
• UniProt Accession #: Q9P209; Q9BV03; Q9BWM3; Q9NVR4; B4DR26
• Molecular Weight: 21,336 Da
• NCBI Official Full Name: Homo sapiens centrosomal protein 72kDa, mRNA
• NCBI Official Synonym Full Names: centrosomal protein 72
• NCBI Official Symbol: CEP72
• NCBI Protein Information: centrosomal protein of 72 kDa
• UniProt Protein Name: Centrosomal protein of 72 kDa
• Protein Family: Centrosomal protein
• UniProt Gene Name: CEP72
• UniProt Synonym Gene Names: KIAA1519
• UniProt Entry Name: CEP72_HUMAN

NCBI Description

The product of this gene is a member of the leucine-rich-repeat (LRR) superfamily of proteins. The protein is localized to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. [provided by RefSeq, Jul 2008]