Rabbit anti-Human CEP57 Polyclonal Antibody | anti-CEP57 antibody

CEP57, NT (CEP57, KIAA0092, TSP57, Centrosomal protein of 57kD, FGF2-interacting protein, Testis-specific protein 57, Translokin) (APC)

Cat. Num. AAA6285173

• Gene Names: CEP57; MVA2; PIG8; TSP57
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CEP57; Polyclonal Antibody; NT (CEP57; KIAA0092; TSP57; Centrosomal protein of 57kD; FGF2-interacting protein; Testis-specific protein 57; Translokin) (APC); Translokin; anti-CEP57 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CEP57 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CEP57 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the N-terminal region of human CEP57.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CEP57 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CEP57 antibody detected the CEP57 protein (arrow).)

Related Product Information for anti-CEP57 antibody

Translokin binds basic fibroblast growth factor (FGF2; MIM 134920) and mediates its nuclear translocation and mitogenic activity (Bossard et al., 2003 [PubMed 12717444]).[supplied by OMIM].

Product Categories/Family for anti-CEP57 antibody

Antibodies; Centrosomal Proteins

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Petretti, C., et al. EMBO Rep. 7(4):418-424(2006). Kim, Y.S., et al. Biol. Reprod. 70(1):106-113(2004). Andersen, J.S., et al. Nature 426(6966):570-574(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 45430055
• NCBI GeneID: 9702
• NCBI Accession #: NP_055494.2
• NCBI GenBank Nucleotide #: NM_014679.4
• UniProt Accession #: Q86XR8; Q14704; Q5JB46; Q8IXP0; Q9BVF9; A0PJH1; A8K5D0; B4DDP5; F5H5F7
• Molecular Weight: 56,490 Da
• NCBI Official Full Name: centrosomal protein of 57 kDa isoform a
• NCBI Official Synonym Full Names: centrosomal protein 57kDa
• NCBI Official Symbol: CEP57
• NCBI Official Synonym Symbols: MVA2; PIG8; TSP57
• NCBI Protein Information: centrosomal protein of 57 kDa; translokin; FGF2-interacting protein; testis-specific protein 57; proliferation-inducing protein 8
• UniProt Protein Name: Centrosomal protein of 57 kDa
• Protein Family: Centrosomal protein
• UniProt Gene Name: CEP57
• UniProt Synonym Gene Names: KIAA0092; TSP57; Cep57
• UniProt Entry Name: CEP57_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein called Translokin. This protein localizes to the centrosome and has a function in microtubular stabilization. The N-terminal half of this protein is required for its centrosome localization and for its multimerization, and the C-terminal half is required for nucleating, bundling and anchoring microtubules to the centrosomes. This protein specifically interacts with fibroblast growth factor 2 (FGF2), sorting nexin 6, Ran-binding protein M and the kinesins KIF3A and KIF3B, and thus mediates the nuclear translocation and mitogenic activity of the FGF2. It also interacts with cyclin D1 and controls nucleocytoplasmic distribution of the cyclin D1 in quiescent cells. This protein is crucial for maintaining correct chromosomal number during cell division. Mutations in this gene cause mosaic variegated aneuploidy syndrome, a rare autosomal recessive disorder. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CEP57: Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring- like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2. Defects in CEP57 are the cause of mosaic variegated aneuploidy syndrome type 2 (MVA2). MVA2 is a severe developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. Belongs to the translokin family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; microtubule cytoskeleton; Golgi apparatus; microtubule; centrosome; cytoplasm; nucleus; cytosol

Molecular Function: gamma-tubulin binding; protein binding; fibroblast growth factor binding; protein homodimerization activity; microtubule binding

Biological Process: fibroblast growth factor receptor signaling pathway; protein import into nucleus, translocation; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein homooligomerization; spermatid development

Disease: Mosaic Variegated Aneuploidy Syndrome 2