Goat CENTG3/MRIP1 Polyclonal Antibody | anti-AGAP3 antibody

Goat anti-CENTG3/MRIP1 Antibody

Cat. Num. AAA420354

• Gene Names: AGAP3; CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CENTG3/MRIP1; Polyclonal Antibody; Goat anti-CENTG3/MRIP1 Antibody; AGAP3; CENTG3; MRIP1; centaurin; gamma 3; MRIP-1; MRIP-1 protein; ArfGAP with GTPase domain; ankyrin repeat and PH domain 3; CRAG; FLJ16146; FLJ34452; CRMP (collapsin response mediator protein) associated; AGAP3 antibody; CENTG3 antibody; MRIP1 antibody; gamma 3 antibody; MRIP-1 antibody; MRIP-1 protein antibody; ankyrin repeat and PH domain 3 antibody; CRAG antibody; FLJ16146 antibody; FLJ34452 antibody; CRMP (collapsin response mediator protein) associated antibody; CENTG3 / MRIP1; anti-AGAP3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NPSAELHRSPSLL
• Sequence Length: 911

• Applicable Applications for anti-AGAP3 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10ug/ml.; Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx.101kDda according to NP_114152.
• Immunogen: Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 110227613
• NCBI GeneID: 116988
• NCBI Accession #: NP_114152.3
• NCBI GenBank Nucleotide #: NM_031946.6
• Molecular Weight: 42,517 Da
• NCBI Official Full Name: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform a
• NCBI Official Synonym Full Names: ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
• NCBI Official Symbol: AGAP3
• NCBI Official Synonym Symbols: CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
• NCBI Protein Information: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
• UniProt Protein Name: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
• Protein Family: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein
• UniProt Gene Name: AGAP3
• UniProt Synonym Gene Names: CENTG3; AGAP-3; CRAG; Cnt-g3; MRIP-1
• UniProt Entry Name: AGAP3_HUMAN

Uniprot Description

CENTG3: a member of the centaurin family of ADP-ribosylation factor directed (ARF) GTPase-activating proteins (GAPs). Protective against the pathogenesis of polyglutamine diseases. Mediates the nuclear translocation of the CENTG3-polyQ complexes and the formation of promyelocytic leukemia (PML) protein-associated nuclear bodies in reactive oxygen species (ROS) signaling. Promotes the degradation of polyQ proteins at PML nuclear bodies through the ubiquitin-proteasome pathway. Knockdown by small interfering RNA in neuronal cells consistently blocked the nuclear translocation of polyQ and enhanced polyQ-mediated cell death. Three alternatively-spliced isoforms have been described.

Protein type: GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: cytoplasm; membrane

Molecular Function: GTP binding; GTPase activator activity; metal ion binding

Biological Process: positive regulation of GTPase activity; protein transport; small GTPase mediated signal transduction