Rabbit anti-Human CENPM Polyclonal Antibody | anti-CENPM antibody

CENPM (C22orf18, ICEN39, PANE1, Centromere Protein M, Interphase Centromere Complex Protein 39, Proliferation-associated Nuclear Element Protein 1, MGC861, PANE1, BK250D10.2) (FITC)

Cat. Num. AAA6373667

• Gene Names: CENPM; PANE1; CENP-M; C22orf18
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CENPM; Polyclonal Antibody; CENPM (C22orf18; ICEN39; PANE1; Centromere Protein M; Interphase Centromere Complex Protein 39; Proliferation-associated Nuclear Element Protein 1; MGC861; BK250D10.2) (FITC); anti-CENPM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CENPM.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 180

• Applicable Applications for anti-CENPM antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CENPM, aa1-180 (NP_076958.1).
• Immunogen Sequence: MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CENPM expression in transfected 293T cell line by CENPM polyclonal antibody. Lane 1: CENPM transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CENPM antibody

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).

Product Categories/Family for anti-CENPM antibody

Antibodies; Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 13129022
• NCBI GeneID: 79019
• NCBI Accession #: NP_076958.1
• NCBI GenBank Nucleotide #: NM_024053
• UniProt Accession #: Q9NSP4
• NCBI Official Full Name: centromere protein M isoform a
• NCBI Official Synonym Full Names: centromere protein M
• NCBI Official Symbol: CENPM
• NCBI Official Synonym Symbols: PANE1; CENP-M; C22orf18
• NCBI Protein Information: centromere protein M
• UniProt Protein Name: Centromere protein M
• Protein Family: Centromere protein
• UniProt Gene Name: CENPM
• UniProt Synonym Gene Names: C22orf18; ICEN39; PANE1; CENP-M
• UniProt Entry Name: CENPM_HUMAN

NCBI Description

The protein encoded by this gene is an inner protein of the kinetochore, the multi-protein complex that binds spindle microtubules to regulate chromosome segregation during cell division. It belongs to the constitutive centromere-associated network protein group, whose members interact with outer kinetochore proteins and help to maintain centromere identity at each cell division cycle. The protein is structurally related to GTPases but cannot bind guanosine triphosphate. A point mutation that affects interaction with another constitutive centromere-associated network protein, CENP-I, impairs kinetochore assembly and chromosome alignment, suggesting that it is required for kinetochore formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

CENPM: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; cytosol

Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle