Rabbit anti-Human CENPH Polyclonal Antibody | anti-CENPH antibody

CENPH, ID (CENPH, ICEN35, Centromere protein H, Interphase centromere complex protein 35) (MaxLight 650)

Cat. Num. AAA6285103

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CENPH; Polyclonal Antibody; ID (CENPH; ICEN35; Centromere protein H; Interphase centromere complex protein 35) (MaxLight 650); Interphase centromere complex protein 35; anti-CENPH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CENPH antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CENPH antibody is generated from rabbits immunized with a KLH conjugated Synthetic peptide selected from the center region of human CENPH.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-CENPH antibody

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Product Categories/Family for anti-CENPH antibody

Antibodies; Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 12597655
• NCBI GeneID: 64946
• NCBI Accession #: NP_075060.1
• NCBI GenBank Nucleotide #: NM_022909.3
• UniProt Accession #: Q9H3R5; A8K3Y1
• Molecular Weight: 28,481 Da
• NCBI Official Full Name: centromere protein H
• NCBI Official Synonym Full Names: centromere protein H
• NCBI Official Symbol: CENPH
• NCBI Protein Information: centromere protein H; CENP-H; kinetochore protein CENP-H; interphase centromere complex protein 35
• UniProt Protein Name: Centromere protein H
• Protein Family: Centromere protein
• UniProt Gene Name: CENPH
• UniProt Synonym Gene Names: ICEN35; CENP-H
• UniProt Entry Name: CENPH_HUMAN

NCBI Description

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: nucleoplasm; kinetochore; nucleus; cytosol

Molecular Function: kinetochore binding; protein binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle; kinetochore organization and biogenesis