Rabbit CELF-2 Polyclonal Antibody | anti-CELF-2 antibody

CELF-2 Rabbit pAb

Cat. Num. AAA4756223

• Gene Names: CELF2; ETR3; ETR-3; NAPOR; CUGBP2; BRUNOL3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: CELF-2; Polyclonal Antibody; CELF-2 Rabbit pAb; CUGBP Elav-like family member 2; ETR3; ETR-3; NAPOR; CUGBP2; BRUNOL3; CUG-BP2; anti-CELF-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-CELF-2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000; IHC: 1:20; ICC/IF: 1:50; FC/FACS: 1:50; IP: 1:20
• Immunogen: Recombinant protein of human CELF-2
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of CELF-2 in HepG2,293, Hela,K562 cell lysates using CELF-2 Rabbit pAb(1:1000 diluted).Predicted band size:54kDa.Observed band size:54kDa.)

Related Product Information for anti-CELF-2 antibody

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA (PubMed:28431233).
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Product Categories/Family for anti-CELF-2 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 134152680
• NCBI GeneID: 10659
• NCBI Accession #: NP_006552.3
• NCBI GenBank Nucleotide #: NM_006561.3
• UniProt Accession #: O95319; Q7KYU4; Q8N499; Q92950; Q96NW9; Q96RQ5; Q96RQ6; Q9UL67; B7ZAN9
• Molecular Weight: 52,054 Da
• NCBI Official Full Name: CUGBP Elav-like family member 2 isoform 2
• NCBI Official Synonym Full Names: CUGBP, Elav-like family member 2
• NCBI Official Symbol: CELF2
• NCBI Official Synonym Symbols: ETR3; ETR-3; NAPOR; CUGBP2; BRUNOL3
• NCBI Protein Information: CUGBP Elav-like family member 2; CELF-2; CUG-BP2; bruno-like protein 3; RNA-binding protein BRUNOL-3; CUG-BP- and ETR-3-like factor 2; ELAV-type RNA-binding protein 3; CUG triplet repeat RNA-binding protein 2; neuroblastoma apoptosis-related RNA-binding p
• UniProt Protein Name: CUGBP Elav-like family member 2
• UniProt Gene Name: CELF2
• UniProt Synonym Gene Names: BRUNOL3; CUGBP2; ETR3; NAPOR; CELF-2; CUG-BP2; ETR-3; hNAPOR
• UniProt Entry Name: CELF2_HUMAN

NCBI Description

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CELF2: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. Belongs to the CELF/BRUNOL family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleus

Molecular Function: RNA binding; nucleotide binding

Biological Process: RNA processing; mRNA processing; regulation of heart contraction