Rabbit anti-Human CELA3B Polyclonal Antibody | anti-CELA3B antibody

CELA3B, NT (CELA3B, ELA3B, Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E) (Azide free) (HRP)

Cat. Num. AAA6285044

• Gene Names: CELA3B; E1; CBPP; EL-1; ELA3B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CELA3B; Polyclonal Antibody; NT (CELA3B; ELA3B; Chymotrypsin-like elastase family member 3B; Elastase IIIB; Elastase-3B; Protease E) (Azide free) (HRP); Protease E; anti-CELA3B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CELA3B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CELA3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human CELA3B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6005083 in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CELA3B antibody detected the CELA3B protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6005083 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-CELA3B antibody

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Product Categories/Family for anti-CELA3B antibody

Antibodies; Elastase

References

Gao, J., et al. Oncol. Rep. 23(6):1683-1692(2010). Teichmann, J., et al. Eur. J. Med. Res. 13(12):563-567(2008). Hahn, J.U., et al. Pancreas 36(3):274-278(2008). Mann, S.T., et al. Eur. J. Med. Res. 13(2):68-72(2008). Vesterhus, M., et al. Diabetes Care 31(2):306-310(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 6679625
• NCBI GeneID: 23436
• NCBI Accession #: NP_031378.1
• NCBI GenBank Nucleotide #: NM_007352.2
• UniProt Accession #: P08861; P11423; Q5VU28; Q5VU29; Q5VU30; B2RE44
• Molecular Weight: Antigen: ~17kDa
• NCBI Official Full Name: chymotrypsin-like elastase family member 3B preproprotein
• NCBI Official Synonym Full Names: chymotrypsin like elastase family member 3B
• NCBI Official Symbol: CELA3B
• NCBI Official Synonym Symbols: E1; CBPP; EL-1; ELA3B
• NCBI Protein Information: chymotrypsin-like elastase family member 3B
• UniProt Protein Name: Chymotrypsin-like elastase family member 3B
• Protein Family: Chymotrypsin-like elastase family
• UniProt Gene Name: CELA3B
• UniProt Synonym Gene Names: ELA3B
• UniProt Entry Name: CEL3B_HUMAN