Western Blot (WB)
(Western blot analysis using FAK mouse mAb against A549 (1) and NIH/3T3 (2) cell lysate.)
Mouse anti-Human, Mouse FAK Monoclonal Antibody | anti-FAK antibody
Anti-FAK Mouse mAb
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
FAK; Monoclonal Antibody; Anti-FAK Mouse mAb; PI3K; PIK3; PI3CG; PI3Kgamma; p110gamma; p120-PI3K; anti-FAK antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4B5D2
Form/Format
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Applicable Applications for anti-FAK antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Immunogen
Purified recombinant fragment of human PIK3CG (AA: 1-100) expressed in E Coli.
Western Blot (WB)
(Western blot analysis using FAK mouse mAb against A549 (1) and NIH/3T3 (2) cell lysate.)
Western Blot (WB)
(Western blot analysis using FAK mouse mAb against A549 (1) and NIH/3T3 (2) cell lysate.)
Immunofluorescence (IF)
(Immunofluorescence analysis of B16 cells using FAK mouse mAb (green). Blue)
Immunofluorescence (IF)
(Immunofluorescence analysis of B16 cells using FAK mouse mAb (green). Blue)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded cervices tumour using FAK mouse mAb with DAB staining)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded cervices tumour using FAK mouse mAb with DAB staining)
Related Product Information for anti-FAK antibody
Entrez Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.RD: Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.
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Product Notes
The FAK (Catalog #AAA476625) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-FAK Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the FAK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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