Rabbit anti-Human CEBP gamma Polyclonal Antibody | anti-CEBPG antibody

Anti-CEBP gamma Antibody, Rabbit Polyclonal

Cat. Num. AAA8107500

• Gene Names: CEBPG; GPE1BP; IG/EBP-1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CEBP gamma; Polyclonal Antibody; Anti-CEBP gamma Antibody; Rabbit Polyclonal; CEBPG/CEBP gamma Antibody; Rabbit PAb; Antigen Affinity Purified; CCAAT/enhancer binding protein (C/EBP); gamma; Anti-GPE1BP Antibody; Anti-IG/EBP-1 Antibody; anti-CEBPG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CEBPG/CEBP gamma
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CEBPG antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human CEBPG/CEBP gamma Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CEBPG/CEBP gamma (rh CEBPG/CEBP gamma). CEBPG/CEBP gamma specific IgG was purified by Human CEBPG/CEBP gamma affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(CEBPG was immunoprecipitated using:Lane A:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-CEBPG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CEBPG rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)

Western Blot (WB)

(Anti-CEBPG rabbit polyclonal antibody at 1:500 dilutionLane A: Raw264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:16 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CEBPG antibody

CEBPG, also known as CEBP gamma, is a transcription factor which belongs to the CEBP family. Members of this family regulate viral and cellular CCAAT/enhancer element-mediated transcription. CEBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. CEBPG binds to the enhancer element PRE-I of the IL-4 gene. It Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites.

References

Thomassin H. et al., 1992, Nucleic Acids Res. 20 (12): 3091-8. Nishizawa M. et al., 1992, FEBS Lett. 299 (1): 36-8. Williams SC. et al., 1991, Genes Dev. 5 (9): 1553-67. Melchionna R. et al., 2012, J Invest Dermatol. 132 (7): 1908-17.

NCBI and Uniprot Product Information

• NCBI GI #: 356582519
• NCBI GeneID: 1054
• NCBI Accession #: NP_001239225.1
• NCBI GenBank Nucleotide #: NM_001252296.1
• UniProt Accession #: P53567; Q5U052; B2R946
• Molecular Weight: 16,408 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein gamma
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), gamma
• NCBI Official Symbol: CEBPG
• NCBI Official Synonym Symbols: GPE1BP; IG/EBP-1
• NCBI Protein Information: CCAAT/enhancer-binding protein gamma; c/EBP gamma
• UniProt Protein Name: CCAAT/enhancer-binding protein gamma
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPG
• UniProt Synonym Gene Names: C/EBP gamma
• UniProt Entry Name: CEBPG_HUMAN

NCBI Description

The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

C/EBP-gamma: Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites. Belongs to the bZIP family. C/EBP subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription factor binding

Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; natural killer cell mediated cytotoxicity; negative regulation of transcription factor activity; enucleate erythrocyte differentiation; liver development; mRNA metabolic process; regulation of transcription from RNA polymerase II promoter; B cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of interferon-gamma biosynthetic process; positive regulation of DNA binding