Rabbit anti-Human CEACAM Polyclonal Antibody | anti-CEACAM antibody

CEACAM, NT (Carcinoembryonic Antigen-related Cell Adhesion Molecule 1, Biliary Glycoprotein 1, BGP-1, CD66a, BGP1, BGP) (PE)

Cat. Num. AAA6467151

• Gene Names: CEACAM1; BGP; BGP1; BGPI
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CEACAM; Polyclonal Antibody; NT (Carcinoembryonic Antigen-related Cell Adhesion Molecule 1; Biliary Glycoprotein 1; BGP-1; CD66a; BGP1; BGP) (PE); anti-CEACAM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CEACAM.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CEACAM antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CEACAM (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CEACAM antibody (N-term) in HepG2 cell line lysates (35ug/lane). CEACAM (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CEACAM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using CEACAM Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CEACAM antibody

Antibodies; Cancer Markers

References

1. Gambichler, T., Am. J. Clin. Pathol. 131(6), 782-787 (2009). 2. Li, C., Exp. Cell Res. 315(7), 1225-1233 (2009). 3. Nouvion, A.L., Med Sci (Paris) 25(3), 247-252 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 19923195
• NCBI GeneID: 634
• NCBI Accession #: NP_001703
• NCBI GenBank Nucleotide #: NM_001712.5
• UniProt Accession #: P13688
• Molecular Weight: 57kDa
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 1 isoform 1
• NCBI Official Synonym Full Names: carcinoembryonic antigen related cell adhesion molecule 1
• NCBI Official Symbol: CEACAM1
• NCBI Official Synonym Symbols: BGP; BGP1; BGPI
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 1
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 1
• Protein Family: CEACAM1-like protein
• UniProt Gene Name: CEACAM1
• UniProt Synonym Gene Names: BGP; BGP1; BGP-1
• UniProt Entry Name: CEAM1_HUMAN

NCBI Description

This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined. [provided by RefSeq, May 2010]

Uniprot Description

CEACAM1: carcinoembryonic antigen-related cell adhesion molecule 1. A cell-cell adhesion molecule that directly associates with annexin II. Has tumor suppressor activity in prostate carcinoma. Belongs to the immunoglobulin superfamily and the CEA family. Abundantly expressed in epithelia, vessel endothelia, trophoblasts, platelets and neutrophilic granulocytes. Also present in many cell types of the immune system such as B cells, T cells, NK cells, macrophages and dendritic cells. Five alternatively spliced isoforms have been described. Two isoforms are type I membrane proteins, while three are secreted.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Immunoglobulin superfamily; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; protein homodimerization activity

Biological Process: integrin-mediated signaling pathway; cell migration; angiogenesis; homophilic cell adhesion; blood coagulation; leukocyte migration