Rabbit anti-Human CEA Polyclonal Antibody | anti-CEA antibody

CEA, ID (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5) (AP)

Cat. Num. AAA6467152

• Gene Names: CEACAM5; CEA; CD66e
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CEA; Polyclonal Antibody; ID (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen; Meconium Antigen 100; CD66e; CEACAM5) (AP); anti-CEA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CEA.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CEA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CEA (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CEA antibody (Center) in 293 cell line lysates (35ug/lane). CEA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CEA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CEA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CEA Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CEA antibody

Antibodies; Cancer Markers

References

1. Lagoudianakis, E.E., J. Surg. Res. 155(2), 183-190 (2009). 2. Gambichler, T., Am. J. Clin. Pathol. 131(6), 782-787 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 98986445
• NCBI GeneID: 1048
• NCBI Accession #: NP_004354
• NCBI GenBank Nucleotide #: NM_004363.3
• UniProt Accession #: P06731; H9KVA7
• Molecular Weight: Predicted: 77 kDa; ; Observed: 80kDa
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 5 preproprotein
• NCBI Official Synonym Full Names: carcinoembryonic antigen-related cell adhesion molecule 5
• NCBI Official Symbol: CEACAM5
• NCBI Official Synonym Symbols: CEA; CD66e
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 5
• Protein Family: Colicin
• UniProt Gene Name: CEACAM5
• UniProt Synonym Gene Names: CEA; CEA
• UniProt Entry Name: CEAM5_HUMAN

NCBI Description

Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]

Uniprot Description

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis