Rabbit anti-Human CDX2 Polyclonal Antibody | anti-CDX2 antibody

CDX2, Phosphorylated (S283) (CDX2, CDX3, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2) (AP)

Cat. Num. AAA6284842

• Gene Names: CDX2; CDX3; CDX-3
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDX2; Polyclonal Antibody; Phosphorylated (S283) (CDX2; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2) (AP); Caudal-type homeobox protein 2; anti-CDX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CDX2 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-CDX2-S283 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 268~297 amino acids surrounding S283 of human CDX2.
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Phospho-CDX2-S283 Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-CDX2 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are0.5ug per ml.)

Related Product Information for anti-CDX2 antibody

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription.

Product Categories/Family for anti-CDX2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Benoit, Y.D., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 298 (4), G504-G517 (2010). Xie, Y., et al. Int. J. Oncol. 36(2):509-516(2010). Park do, Y., et al. Mod. Pathol. 23(1):54-61(2010). Lora, V., et al. Anticancer Res. 29(12):5033-5037(2009). Porjazova, E., et al. Akush Ginekol (Sofiia) 48(4):32-34(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 372624392
• NCBI GeneID: 1045
• NCBI Accession #: NP_001256.3
• NCBI GenBank Nucleotide #: NM_001265.4
• UniProt Accession #: Q99626; O00503; Q5VTU7; Q969L8; Q9UD92
• Molecular Weight: 33,520 Da
• NCBI Official Full Name: homeobox protein CDX-2
• NCBI Official Synonym Full Names: caudal type homeobox 2
• NCBI Official Symbol: CDX2
• NCBI Official Synonym Symbols: CDX3; CDX-3
• NCBI Protein Information: homeobox protein CDX-2; caudal-type homeobox protein 2; caudal type homeobox transcription factor 2; caudal type homeo box transcription factor 2
• UniProt Protein Name: Homeobox protein CDX-2
• Protein Family: Homeobox protein
• UniProt Gene Name: CDX2
• UniProt Synonym Gene Names: CDX3
• UniProt Entry Name: CDX2_HUMAN

NCBI Description

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]

Uniprot Description

CDX2: Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Belongs to the Caudal homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; condensed nuclear chromosome; transcriptional repressor complex

Molecular Function: double-stranded DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of epithelial cell polarity; anterior/posterior pattern formation; blood vessel development; organ morphogenesis; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; endosome to lysosome transport; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; trophectodermal cell differentiation