Rabbit anti-Human Cdx1 Polyclonal Antibody | anti-Cdx1 antibody

Cdx1 Polyclonal Antibody

Cat. Num. AAA3012466

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: ProA affinity purified
• Synonyms: Cdx1; Polyclonal Antibody; Cdx1 Polyclonal Antibody; CDX1; Caudal type homeo box transcription factor 1; Caudal type homeobox 1; Caudal type homeobox protein; Caudal type homeobox protein CDX1; Caudal type homeobox transcription factor 1; Caudal-type homeobox protein 1; Homeobox protein CDX 1; Homeobox protein CDX-1; MGC116915; OTTHUMP00000160524; anti-Cdx1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cdx1 polyclonal antibody detects endogenous levels of Cdx1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 265

• Applicable Applications for anti-Cdx1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200
• Immunogen: Recombinant protein of full lenth sequence of human Cdx1.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Cdx1 on human small intestine tissue (1) and CRC cell (2) lysate using anti-Cdx1 antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining Cdx1 in HOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Cdx1 antibody

The members of the murine Cdx family (Cdx1, Cdx2, and Cdx4) are members of the caudal-type homeobox family of genes, which are homologues of the Drosophila 'caudal' gene required for anterior-posterior regional identity. The intestine-specific transcription factors Cdx1 and Cdx2 are candidate genes for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. The relative expression of Cdx1 to Cdx2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1 positively regulates its own expression. Cdx1 and Cdx2 are expressed in the small intestine and colon of fetus and adult. A decrease in human Cdx1 and/or Cdx2 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways. The human Cdx1 gene maps to chromosome 5q31-q33 and encodes a 265-amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 121114296
• NCBI GeneID: 1044
• NCBI Accession #: NP_001795.2
• NCBI GenBank Nucleotide #: NP_001795.2
• UniProt Accession #: P47902
• Molecular Weight: 28kDa
• NCBI Official Full Name: homeobox protein CDX-1
• NCBI Official Synonym Full Names: caudal type homeobox 1
• NCBI Official Symbol: CDX1
• NCBI Protein Information: homeobox protein CDX-1
• UniProt Protein Name: Homeobox protein CDX-1
• Protein Family: Homeobox protein
• UniProt Gene Name: CDX1

NCBI Description

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. It has been shown to induce expression of the intestinal alkaline phosphatase gene, and inhibit beta-catenin/T-cell factor transcriptional activity. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a role in transcriptional regulation (PubMed:24623306). Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the PRKD1 promoter in colorectal cancer (CRC) cells (PubMed:24623306). Could play a role in the terminal differentiation of the intestine.