papillomavirus type 16 Regulatory protein E2 (E2) Recombinant Protein | E2 recombinant protein

Recombinant Human papillomavirus type 16 Regulatory protein E2 (E2)

Cat. Num. AAA1155141

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: papillomavirus type 16 Regulatory protein E2 (E2); Recombinant Human papillomavirus type 16 Regulatory protein E2 (E2); E2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-365aa; Full Length
• Sequence: METLCQRLNVCQDKILTHYENDSTDLRDHIDYWKHMRLECAIYYKAREMGFKHINHQVVPTLAVSKNKALQAIELQLTLETIYNSQYSNEKWTLQDVSLEVYLTAPTGCIKKHGYTVEVQFDGDICNTMHYTNWTHIYICEEASVTVVEGQVDYYGLYYVHEGIRTYFVQFKDDAEKYSKNKVWEVHAGGQVILCPTSVFSSNEVSSPEIIRQHLANHPAATHTKAVALGTEETQTTIQRPRSEPDTGNPCHTTKLLHRDSVDSAPILTAFNSSHKGRINCNSNTTPIVHLKGDANTLKCLRYRFKKHCTLYTAVSSTWHWTGHNVKHKSAIVTLTYDSEWQRDQFLSQVKIPKTITVSTGFMSI

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for E2 recombinant protein

Plays an accessory role in initiation of DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is roved from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.

Product Categories/Family for E2 recombinant protein

Epigenetics and Nuclear Signaling

References

Human papillomavirus type 16 DNA sequence.Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W.G.Virology 145:181-185(1985) Cloning and sequencing of non-European human papillomavirus (HPV)variant complete genomes from cervicovaginal cells by an overlapping PCR method.Terai M., Fu L., Ma Z., Burk R.D. Transcriptional trans-activation by the human papillomavirus type 16 E2 gene product.Phelps W.C., Howley P.M.J. Virol. 61:1630-1638(1987) Transient replication of human papillomavirus DNAs.Del Vecchio A.M., Romanczuk H., Howley P.M., Baker C.C.J. Virol. 66:5949-5958(1992) Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2.Okoye A., Cordano P., Taylor E.R., Morgan I.M., Everett R., Campo M.S.Virus Res. 108:1-14(2005) Regulation of human papillomavirus type 16 E7 activity through direct protein interaction with the E2 transcriptional activator.Gammoh N., Grm H.S., Massimi P., Banks L.J. Virol. 80:1787-1797(2006) TAF1 interacts with and modulates human papillomavirus 16 E2-dependent transcriptional regulation.Centeno F., Ramirez-Salazar E., Garcia-Villa E., Gariglio P., Garrido E.Intervirology 51:137-143(2008) Expression, crystallization and preliminary X-ray analysis of the E2 transactivation domain from papillomavirus type 16.Burns J.E., Moroz O.V., Antson A.A., Sanders C.M., Wilson K.S., Maitland N.J.Acta Crystallogr. D 54:1471-1474(1998)

NCBI and Uniprot Product Information

• NCBI GI #: 9627106
• NCBI GeneID: 1489080
• NCBI Accession #: NP_041328.1
• NCBI GenBank Nucleotide #: NC_001526.2
• UniProt Accession #: P03120; Q71BI4; W2WLHS
• Molecular Weight: 68.8 kDa
• NCBI Official Full Name: regulatory protein
• NCBI Official Symbol: E2
• NCBI Protein Information: regulatory protein
• UniProt Protein Name: Regulatory protein E2
• Protein Family: Regulatory protein
• UniProt Gene Name: E2
• UniProt Entry Name: VE2_HPV16

Uniprot Description

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.