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Western Blot (WB) (The anti-CDX1 C-term Pab is used in Western blot to detect CDX1 in HL60 cell lysate.)

Rabbit anti-Human CDX1 Polyclonal Antibody | anti-CDX1 antibody

CDX1 Antibody (C-term)

Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CDX1; Polyclonal Antibody; CDX1 Antibody (C-term); Homeobox protein CDX-1; Caudal-type homeobox protein 1; anti-CDX1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the C-terminal region of human CDX1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
236-265
Sequence Length
265
Applicable Applications for anti-CDX1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-CDX1 C-term Pab is used in Western blot to detect CDX1 in HL60 cell lysate.)

Western Blot (WB) (The anti-CDX1 C-term Pab is used in Western blot to detect CDX1 in HL60 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-CDX1 antibody
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. It has been shown to induce expression of the intestinal alkaline phosphatase gene, and inhibit beta-catenin/T-cell factor transcriptional activity.
References
Domon-Dell, C., et al., Oncogene 22(39):7913-7921 (2003).
Suh, E.R., et al., J. Biol. Chem. 277(39):35795-35800 (2002).
Mizoshita, T., et al., Gastric Cancer 4(4):185-191 (2001).
Mallo, G.V., et al., Int. J. Cancer 74(1):35-44 (1997).
Bonner, C.A., et al., Genomics 28(2):206-211 (1995).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28138
NCBI Official Full Name
homeobox protein CDX-1
NCBI Official Synonym Full Names
caudal type homeobox 1
NCBI Official Symbol
CDX1
NCBI Protein Information
homeobox protein CDX-1
UniProt Protein Name
Homeobox protein CDX-1
Protein Family
UniProt Gene Name
CDX1
UniProt Entry Name
CDX1_HUMAN

NCBI Description

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. It has been shown to induce expression of the intestinal alkaline phosphatase gene, and inhibit beta-catenin/T-cell factor transcriptional activity. [provided by RefSeq, Jul 2008]

Uniprot Description

CDX1: Could play a role in the terminal differentiation of the intestine. Belongs to the Caudal homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: nucleus

Molecular Function: protein binding

Biological Process: anterior/posterior pattern formation; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter

Research Articles on CDX1

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Product Notes

The CDX1 cdx1 (Catalog #AAA9207642) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 236-265. The CDX1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CDX1 cdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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