Rabbit anti-Human, Mouse CDKL5 Polyclonal Antibody | anti-CDKL5 antibody

CDKL5, CT (CDKL5, STK9, Cyclin-dependent kinase-like 5, Serine/threonine-protein kinase 9) (PE)

Cat. Num. AAA6284599

• Gene Names: CDKL5; ISSX; STK9; EIEE2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CDKL5; Polyclonal Antibody; CT (CDKL5; STK9; Cyclin-dependent kinase-like 5; Serine/threonine-protein kinase 9) (PE); Serine/threonine-protein kinase 9; anti-CDKL5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CDKL5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDKL5 (STK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 990~1019 amino acids from the C-terminal region of human STK9.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-STK9 Pab is used in Western blot to detect STK9 in mouse lung tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with STK9 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-CDKL5 antibody

Defects in STK9, a dual-specificity serine/threonine kinase, are a cause of atypical Rett syndrome. Rett syndrome is an X-linked dominant disease. It is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Patients appear to develop normally until 6 to 18 months of age, then gradually lose speech and purposeful hand movements and develop microcephaly, seizures, autism, ataxia, intermittent hyperventilation, and stereotypic hand movements. After initial regression, the condition stabilizes and patients usually survive into adulthood. Rett syndrome due to CDKL5-associated mutations is characterized by a severe early-onset phenotype and atypical features such as infantile spasms.

Product Categories/Family for anti-CDKL5 antibody

Antibodies; Protein Kinases

References

Kalscheuer, V.M., et al., Am. J. Hum. Genet. 72(6):1401-1411 (2003). Montini, E., et al., Genomics 51(3):427-433 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 83367066
• NCBI GeneID: 6792
• NCBI Accession #: NP_003150.1
• NCBI GenBank Nucleotide #: NM_003159
• UniProt Accession #: O76039; Q14198; Q5H985; Q8IYC7; Q9UJL6; G9B9X4
• Molecular Weight: ~84 kDa
• NCBI Official Full Name: Homo sapiens cyclin-dependent kinase-like 5 (CDKL5), transcript variant I, mRNA
• NCBI Official Synonym Full Names: cyclin-dependent kinase-like 5
• NCBI Official Symbol: CDKL5
• NCBI Official Synonym Symbols: ISSX; STK9; EIEE2
• NCBI Protein Information: cyclin-dependent kinase-like 5; serine/threonine kinase 9; serine/threonine-protein kinase 9; cyclin dependent kinase 5 transcript
• UniProt Protein Name: Cyclin-dependent kinase-like 5
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDKL5
• UniProt Synonym Gene Names: STK9
• UniProt Entry Name: CDKL5_HUMAN

NCBI Description

This gene is a member of Ser/Thr protein kinase family and encodes a phosphorylated protein with protein kinase activity. Mutations in this gene have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Mediates phosphorylation of MECP2. Ref.7 Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts with MECP2. Ref.7

Subcellular location: Nucleus Ref.8.

Tissue specificity: Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis.

Post-translational modification: Autophosphorylated. Ref.7 Ref.8

Involvement in disease: Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).Epileptic encephalopathy, early infantile, 2 (EIEE2) [MIM:300672]: A severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients with epileptic encephalopathy early infantile type 2 manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements. However, EIEE2 and Rett syndrome are considered two distinct entities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.6 Ref.7 Ref.8 Ref.13 Ref.14 Ref.15 Ref.16 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.

Caution: It is uncertain whether Met-1 or Met-10 is the initiator.

Sequence caution: The sequence CAA61445.1 differs from that shown. Reason: Frameshift at position 415.