Western Blot (WB)
(All lanes : Anti-Cdk7 Antibody (T170) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human CDK7 Polyclonal Antibody | anti-CDK7 antibody
CDK7 Antibody (Center)
Western Blot (WB)
(All lanes : Anti-Cdk7 Antibody (T170) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Cdk7 Antibody (T170) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Background: Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin- dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008]
Uniprot Description
CDK7: a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle. Phosphorylates and activates RNA polymerase II, allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Protein type: EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; EC 2.7.11.23; Protein kinase, CMGC; Cell cycle regulation; Kinase, protein; CMGC group; CDK family; CDK/CDK7 subfamily; CDK7 subfamily
Chromosomal Location of Human Ortholog: 5q12.1
Cellular Component: cytoplasm; holo TFIIH complex; mitochondrion; nucleoplasm; nucleus; perinuclear region of cytoplasm
Molecular Function: androgen receptor binding; ATP binding; cyclin-dependent protein kinase activity; DNA-dependent ATPase activity; kinase activity; protein binding; protein C-terminus binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity; transcription coactivator activity
Biological Process: androgen receptor signaling pathway; cell cycle arrest; cell division; cell proliferation; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; mRNA capping; nucleotide-excision repair, preincision complex assembly; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cyclin-dependent protein kinase activity; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair
Research Articles on CDK7
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Product Notes
The CDK7 cdk7 (Catalog #AAA9217138) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK7 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:1000. Researchers should empirically determine the suitability of the CDK7 cdk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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