Rabbit anti-Human, Mouse Cdk6 Polyclonal Antibody | anti-Cdk6 antibody

Anti-Cdk6 Antibody

Cat. Num. AAA417311

• Gene Names: CDK6; MCPH12; PLSTIRE
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: Cdk6; Polyclonal Antibody; Anti-Cdk6 Antibody; Anti-CDK6 (phospho-Tyr24) Antibody; anti-Cdk6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CDK6 (phospho-Tyr24) Antibody detects endogenous levels of CDK6 only when phosphorylated at tyrosine 24.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 326

• Applicable Applications for anti-Cdk6 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50~100; IF:1:100~1:200
• Storage Buffer: Rabbit IgG in phosphate buffered saline (without Mg< sup>2< /sup>+ and Ca< sup>2< /sup>+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Immunogen: The antibody was produced against synthesized phosphopeptide derived from human CDK6 around the phosphorylation site of tyrosine 24 (G-A-YP-G-K).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-Cdk6 antibody

This gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

NCBI and Uniprot Product Information

• NCBI GI #: 223718134
• NCBI GeneID: 1021
• NCBI Accession #: NP_001138778.1
• NCBI GenBank Nucleotide #: NM_001145306.1
• Molecular Weight: 36,938 Da
• NCBI Official Full Name: cyclin-dependent kinase 6
• NCBI Official Synonym Full Names: cyclin-dependent kinase 6
• NCBI Official Symbol: CDK6
• NCBI Official Synonym Symbols: MCPH12; PLSTIRE
• NCBI Protein Information: cyclin-dependent kinase 6; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE
• UniProt Protein Name: Cyclin-dependent kinase 6
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK6
• UniProt Entry Name: CDK6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

Uniprot Description

CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; Cell cycle regulation; EC 2.7.11.22; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: nucleoplasm; ruffle; centrosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleus

Molecular Function: protein binding; cyclin binding; cyclin-dependent protein kinase activity; ATP binding

Biological Process: Notch signaling pathway; dentate gyrus development; negative regulation of myeloid cell differentiation; response to virus; gliogenesis; T cell differentiation in the thymus; protein amino acid phosphorylation; astrocyte development; negative regulation of cell cycle; regulation of erythrocyte differentiation; negative regulation of cell proliferation; negative regulation of cell differentiation; positive regulation of fibroblast proliferation; regulation of gene expression; cell division; generation of neurons; cell dedifferentiation; negative regulation of osteoblast differentiation; mitotic cell cycle; positive regulation of cell-matrix adhesion; cell cycle arrest; lateral ventricle development; negative regulation of epithelial cell proliferation; G1/S transition of mitotic cell cycle

Disease: Microcephaly 12, Primary, Autosomal Recessive