Goat anti-Human CDCP1, isoform 1 Polyclonal Antibody | anti-CDCP1 antibody

CDCP1, isoform 1, CT (CUB domain containing protein 1, CD318, Membrane glycoprotein gp140, SIMA135, Subtractive immunization M plus HEp3-associated 135kD protein, Transmembrane and associated with src kinases, TRASK, UNQ2486/PRO5773)

Cat. Num. AAA622157

• Gene Names: CDCP1; CD318; TRASK; SIMA135
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CDCP1; isoform 1; Polyclonal Antibody; CT (CUB domain containing protein 1; CD318; Membrane glycoprotein gp140; SIMA135; Subtractive immunization M plus HEp3-associated 135kD protein; Transmembrane and associated with src kinases; TRASK; UNQ2486/PRO5773); Anti -CDCP1; anti-CDCP1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDCP1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-CDCP1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32000; Western Blot: 1-3ug/ml
• Immunogen: Synthetic peptide corresponding to C-PLLNTQEPMEPAE, from the C Terminus of human CDCP1 (NP_073753.3).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(EB05267 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Testing Data

(HEK293 overexpressing CDCP1 (RC220633) and probed with EB05267 (mock transfection in first lane), tested by Origene.)

Related Product Information for anti-CDCP1 antibody

CDCP1(CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135kD cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa30-667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa270-300. This generates an 80kD TM protein that may be missing the N-terminal CUB domain (aa 221-350). Over aa25-667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

Product Categories/Family for anti-CDCP1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 30410805
• NCBI GeneID: 64866
• NCBI Accession #: NP_073753.3
• NCBI GenBank Nucleotide #: NM_022842.3
• UniProt Accession #: Q9H5V8; Q49UB4; Q6NT71; Q6U9Y2; Q8WU91; Q96QU7; Q9H676; Q9H8C2
• Molecular Weight: 92,932 Da
• NCBI Official Full Name: CUB domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: CUB domain containing protein 1
• NCBI Official Symbol: CDCP1
• NCBI Official Synonym Symbols: CD318; TRASK; SIMA135
• NCBI Protein Information: CUB domain-containing protein 1; OTTHUMP00000164120; OTTHUMP00000209568; membrane glycoprotein gp140; transmembrane and associated with src kinases; subtractive immunization M plus HEp3-associated 135 kDa protein
• UniProt Protein Name: CUB domain-containing protein 1
• Protein Family: CUB domain-containing protein
• UniProt Gene Name: CDCP1
• UniProt Synonym Gene Names: TRASK
• UniProt Entry Name: CDCP1_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Uniprot Description

CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular region; integral to membrane; plasma membrane