Rabbit anti-Human CDCP1 Polyclonal Antibody | anti-CDCP1 antibody

CDCP1, CT (CDCP1, TRASK, CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135kD protein, Transmembrane and associated with src kinases, CD318) (FITC)

Cat. Num. AAA6284053

• Gene Names: CDCP1; CD318; TRASK; SIMA135
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDCP1; Polyclonal Antibody; CT (CDCP1; TRASK; CUB domain-containing protein 1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135kD protein; Transmembrane and associated with src kinases; CD318) (FITC); CD318; anti-CDCP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CDCP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CDCP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 703-732 amino acids from the C-terminal region of human CDCP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CDCP1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CDCP1 antibody detected the CDCP1 protein (arrow).)

Related Product Information for anti-CDCP1 antibody

CDCP1 is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-CDCP1 antibody

Antibodies; Transmembrane Proteins

References

He, Y., et al. J. Biol. Chem. 285(34):26162-26173(2010). Miyazawa, Y., et al. Cancer Res. 70(12):5136-5146(2010). Mamat, S., et al. Oncol. Rep. 23(5):1221-1227(2010). Fukuchi, K., et al. Mol. Pharm. 7(1):245-253(2010). Deryugina, E.I., et al. Mol. Cancer Res. 7(8):1197-1211(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 30410803
• NCBI GeneID: 64866
• NCBI Accession #: NP_835488.1; NP_073753.3
• NCBI GenBank Nucleotide #: NM_178181.2
• UniProt Accession #: Q9H5V8; Q49UB4; Q6NT71; Q6U9Y2; Q8WU91; Q96QU7; Q9H676; Q9H8C2
• Molecular Weight: 92,932 Da
• NCBI Official Full Name: CUB domain-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: CUB domain containing protein 1
• NCBI Official Symbol: CDCP1
• NCBI Official Synonym Symbols: CD318; TRASK; SIMA135
• NCBI Protein Information: CUB domain-containing protein 1; membrane glycoprotein gp140; transmembrane and associated with src kinases; subtractive immunization M plus HEp3-associated 135 kDa protein
• UniProt Protein Name: CUB domain-containing protein 1
• Protein Family: CUB domain-containing protein
• UniProt Gene Name: CDCP1
• UniProt Synonym Gene Names: TRASK; SIMA135
• UniProt Entry Name: CDCP1_HUMAN

Uniprot Description

CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 3p21.31

Molecular Function: protein binding